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WB analysis of HeLa nuclear extract using GTX30619 EP300 antibody [RW109]. Dilution : 1:1000
WB analysis of HeLa nuclear extract using GTX30619 EP300 antibody [RW109]. Dilution : 1:1000
WB analysis of HeLa nuclear extract using GTX30619 EP300 antibody [RW109]. Dilution : 1:1000

EP300 antibody [RW109]

GTX30619
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetEP300
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Overview

  • Supplier
    GeneTex
  • Product Name
    EP300 antibody [RW109]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. ICC/IF: 1:100 - 1:200. IP: 1:10 - 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RW109
  • Conjugate
    Unconjugated
  • Gene ID2033
  • Target name
    EP300
  • Target description
    E1A binding protein p300
  • Target synonyms
    E1A-associated protein p300; E1A-binding protein, 300kD; histone acetyltransferase p300; histone butyryltransferase p300; histone crotonyltransferase p300; KAT3B; MKHK2; p300; p300 HAT; protein 2-hydroxyisobutyryltransferase p300; protein lactyltransferas p300; protein propionyltransferase p300; RSTS2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ09472
  • Protein Name
    Histone acetyltransferase p300
  • Scientific Description
    Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Regulatory mechanisms and function of hypoxia-induced long noncoding RNA NDRG1-OT1 in breast cancer cells. Chao HH et al., 2022 Sep 20, Cell Death Dis
    Read more

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Figure 1. Western blot analysis of KAT3B/P300/EP300 using anti-KAT3B/P300/EP300 antibody (A00117). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAT3B/P300/EP300 antigen affinity purified polyclonal antibody (Catalog # A00117) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KAT3B/P300/EP300 at approximately 264-300 kDa. The expected band size for KAT3B/P300/EP300 is at 264 kDa.
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