
1:1000 (1μg/mL) Ab dilution used in WB of 10μg (1) and 30μg (2) HeLa cell lysates
ERK1/2 antibody [E31R]
GTX82556
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetMAPK1
Overview
- SupplierGeneTex
- Product NameERK1/2 antibody [E31R]
- Delivery Days Customer9
- Application Supplier NoteRecommended Starting Dilutions:WB (with ECL): Use at a dilution of 1:1000For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDE31R
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5594
- Target nameMAPK1
- Target descriptionmitogen-activated protein kinase 1
- Target synonymsERK, ERK-2, ERK2, ERT1, MAPK2, NS13, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk, p42-MAPK, mitogen-activated protein kinase 1, MAP kinase 1, MAP kinase 2, MAPK 2, extracellular signal-regulated kinase 2, mitogen-activated protein kinase 2, protein tyrosine kinase ERK2
- HostMouse
- IsotypeIgG1
- Scientific DescriptionThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Li MY, Chang CT, Han YT, et al. Ginkgolide B promotes neuronal differentiation through the Wnt/β-catenin pathway in neural stem cells of the postnatal mammalian subventricular zone. Sci Rep. 2018,8(1):14947. doi: 10.1038/s41598-018-32960-8Read this paper
- Hsu LH, Liu KJ, Tsai MF, et al. Estrogen adversely affects the prognosis of patients with lung adenocarcinoma. Cancer Sci. 2015,106(1):51-9. doi: 10.1111/cas.12558Read this paper



![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK2 antibody [GT1183] (GTX00945) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX00945/GTX00945_4000000169_20200313_WB_w_23053121_448.webp)



