Fibrillarin antibody [38F3]

GeneTex

Fibrillarin antibody [38F3]

9

IF (ICC): 1/1000 - 1/5000 (yeast cells). For IF of mammalian cells, 1/500. WB: 1/2000 (cell lysates) - 1/10000 (nuclear fractions)(ECL). Detects a band of approximately 34 kDa. For other (non-ECL) western detection methods, 1/1000 - 1/5000. To detect mammalian fibrillarin on western blots by ECL, 1/500. Optimal dilutions/concentrations should be determined by the end user.

ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

38F3

Unconjugated

FBL

fibrillarin

FIB, FLRN, Nop1, RNU3IP1, rRNA 2'-O-methyltransferase fibrillarin, 34 kDa nucleolar scleroderma antigen, 34-kD nucleolar scleroderma antigen, RNA, U3 small nucleolar interacting protein 1, U6 snRNA 2'-O-methyltransferase fibrillarin, histone-glutamine methyltransferase

Mouse

IgG1

rRNA 2'-O-methyltransferase fibrillarin

This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]

C. Elegans, Drosophila, Human, Mouse, Plant, Rat, Yeast

-20°C or -80°C,2°C to 8°C

12352203