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ProductsBack/Fibrillarin + FBLL1 antibody [HL2588]
Fibrillarin + FBLL1 antibody [HL2588] detects Fibrillarin + FBLL1 protein at nuclear envelope and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Fibrillarin + FBLL1 stained by Fibrillarin + FBLL1 antibody [HL2588] (GTX639039) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
Fibrillarin + FBLL1 antibody [HL2588] detects Fibrillarin + FBLL1 protein at nuclear envelope and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Fibrillarin + FBLL1 stained by Fibrillarin + FBLL1 antibody [HL2588] (GTX639039) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
Fibrillarin + FBLL1 antibody [HL2588] detects Fibrillarin + FBLL1 protein at nuclear envelope and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Fibrillarin + FBLL1 stained by Fibrillarin + FBLL1 antibody [HL2588] (GTX639039) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).

Fibrillarin + FBLL1 antibody [HL2588]

GTX639039
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFBL
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Overview

  • Supplier
    GeneTex
  • Product Name
    Fibrillarin + FBLL1 antibody [HL2588]
  • Delivery Days Customer
    7
  • Application Supplier Note
    ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL2588
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2091
  • Target name
    FBL
  • Target description
    fibrillarin
  • Target synonyms
    FIB, FLRN, Nop1, RNU3IP1, rRNA 2'-O-methyltransferase fibrillarin, 34 kDa nucleolar scleroderma antigen, 34-kD nucleolar scleroderma antigen, RNA, U3 small nucleolar interacting protein 1, U6 snRNA 2'-O-methyltransferase fibrillarin, histone-glutamine methyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22087
  • Protein Name
    rRNA 2'-O-methyltransferase fibrillarin
  • Scientific Description
    This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Fibrillarin/FBL using anti-Fibrillarin/FBL antibody (A03178-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human A375 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fibrillarin/FBL antigen affinity purified polyclonal antibody (Catalog # A03178-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fibrillarin/FBL at approximately 37 kDa. The expected band size for Fibrillarin/FBL is at 37 kDa.
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