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Intracellular staining of human peripheral blood cells (gated on CD4+ cells) with anti-FoxP3 (clone 3G3) (GTX80283)
Intracellular staining of human peripheral blood cells (gated on CD4+ cells) with anti-FoxP3 (clone 3G3) (GTX80283)
Intracellular staining of human peripheral blood cells (gated on CD4+ cells) with anti-FoxP3 (clone 3G3) (GTX80283)

FOXP3 antibody [3G3]

GTX80283
GeneTex
ApplicationsFlow Cytometry, Western Blot
DownloadDatasheet
Product group Antibodies
TargetFoxp3
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Overview

  • Supplier
    GeneTex
  • Product Name
    FOXP3 antibody [3G3]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3G3
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID20371
  • Target name
    Foxp3
  • Target description
    forkhead box P3
  • Target synonyms
    forkhead box protein P3; JM2; scu; scurfin; scurfy; sf
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ99JB6
  • Protein Name
    Forkhead box protein P3
  • Scientific Description
    The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene result in the scurfy phenotype (sf). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development. Gavin MA et al., 2006 Apr 25, Proc Natl Acad Sci U S A
    Read more

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FACS analysis of C57Bl/6 splenocytes stained FITC anti-mouse CD4 antibody, followed by intracellular staining with GTX80283-07 FOXP3 antibody [3G3] (APC). Right panel : Co-stained with FITC anti-mouse CD4 antibody and GTX80283-07 Left panel : Co-stained with FITC anti-mouse CD4 antibody and APC mouse IgG1 isotype control Antibody amount : 0.125 microg
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Figure 1. Western blot analysis of Foxp3 using anti-Foxp3 antibody (A00011-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Foxp3 antigen affinity purified polyclonal antibody (Catalog # A00011-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Foxp3 at approximately 50 kDa. The expected band size for Foxp3 is at 50 kDa.
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