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FACS analysis of mouse C57Bl/6 splenocytes using GTX01488-08 FOXP3 antibody [MF23] (PE). Right panel : primary antibody Left panel : isotype control antibody amount : 0.25 microg (5 microl)
FACS analysis of mouse C57Bl/6 splenocytes using GTX01488-08 FOXP3 antibody [MF23] (PE). Right panel : primary antibody Left panel : isotype control antibody amount : 0.25 microg (5 microl)
FACS analysis of mouse C57Bl/6 splenocytes using GTX01488-08 FOXP3 antibody [MF23] (PE). Right panel : primary antibody Left panel : isotype control antibody amount : 0.25 microg (5 microl)

FOXP3 antibody [MF23] (PE)

GTX01488-08
GeneTex
ApplicationsFlow Cytometry
Product group Antibodies
TargetFoxp3
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Overview

  • Supplier
    GeneTex
  • Product Name
    FOXP3 antibody [MF23] (PE)
  • Delivery Days Customer
    9
  • Application Supplier Note
    *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MF23
  • Concentration
    0.2 mg/ml
  • Conjugate
    RPE
  • Gene ID20371
  • Target name
    Foxp3
  • Target description
    forkhead box P3
  • Target synonyms
    JM2, scurfin, sf, forkhead box protein P3, scurfy
  • Host
    Rat
  • Isotype
    IgG2b
  • Protein IDQ99JB6
  • Protein Name
    Forkhead box protein P3
  • Scientific Description
    The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene result in the scurfy phenotype (sf). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • CD25+ T cell depletion impairs murine squamous cell carcinoma development via modulation of antitumor immune responses. Ramos RN et al., 2012 Apr, Carcinogenesis
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  • Repression of cyclic adenosine monophosphate upregulation disarms and expands human regulatory T cells. Klein M et al., 2012 Feb 1, J Immunol
    Read more
  • Blocking of alpha4beta7 gut-homing integrin during acute infection leads to decreased plasma and gastrointestinal tissue viral loads in simian immunodeficiency virus-infected rhesus macaques. Ansari AA et al., 2011 Jan 15, J Immunol
    Read more
  • Epigenetic inheritance of DNA methylation limits activation-induced expression of FOXP3 in conventional human CD25-CD4+ T cells. Nagar M et al., 2008 Aug, Int Immunol
    Read more
  • Effective proliferation of human regulatory T cells requires a strong costimulatory CD28 signal that cannot be substituted by IL-2. Hombach AA et al., 2007 Dec 1, J Immunol
    Read more
  • Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development. Gavin MA et al., 2006 Apr 25, Proc Natl Acad Sci U S A
    Read more

Datasheet

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Figure 1. Western blot analysis of Foxp3 using anti-Foxp3 antibody (A00011-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Foxp3 antigen affinity purified polyclonal antibody (Catalog # A00011-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Foxp3 at approximately 50 kDa. The expected band size for Foxp3 is at 50 kDa.
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