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ProductsBack/gamma Tubulin antibody [GT4511]
gamma Tubulin antibody [GT4511] detects gamma Tubulin protein at centrosome by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: gamma Tubulin stained by gamma Tubulin antibody [GT4511] (GTX629704) diluted at 1:500. Red: alpha Tubulin, stained by alpha Tubulin antibody (GTX112141) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
gamma Tubulin antibody [GT4511] detects gamma Tubulin protein at centrosome by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: gamma Tubulin stained by gamma Tubulin antibody [GT4511] (GTX629704) diluted at 1:500. Red: alpha Tubulin, stained by alpha Tubulin antibody (GTX112141) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
gamma Tubulin antibody [GT4511] detects gamma Tubulin protein at centrosome by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: gamma Tubulin stained by gamma Tubulin antibody [GT4511] (GTX629704) diluted at 1:500. Red: alpha Tubulin, stained by alpha Tubulin antibody (GTX112141) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).

gamma Tubulin antibody [GT4511]

GTX629704
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityDrosophila, Human, Mouse, Rat
TargetTUBG1
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Overview

  • Supplier
    GeneTex
  • Product Name
    gamma Tubulin antibody [GT4511]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT4511
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7283
  • Target name
    TUBG1
  • Target description
    tubulin gamma 1
  • Target synonyms
    CDCBM4, GCP-1, TUBG, TUBGCP1, tubulin gamma-1 chain, gamma-tubulin complex component 1, tubulin, gamma polypeptide
  • Host
    Mouse
  • Isotype
    IgG2b
  • Scientific Description
    This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq]
  • Reactivity
    Drosophila, Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Konno T, Kohno T, Kikuchi S, et al. Localization of Tricellular Tight Junction Molecule LSR at Midbody and Centrosome During Cytokinesis in Human Epithelial Cells. J Histochem Cytochem. 2020,68(1):59-72. doi: 10.1369/0022155419886263
    Read this paper
  • Cheng HW, Hsiao CT, Chen YQ, et al. Centrosome guides spatial activation of Rac to control cell polarization and directed cell migration. Life Sci Alliance. 2019,2(1). doi: 10.26508/lsa.201800135
    Read this paper
  • Okumura T, Takeda K, Taniguchi K, et al. βν integrin inhibits chronic and high level activation of JNK to repress senescence phenotypes in Drosophila adult midgut. PLoS One. 2014,9(2):e89387. doi: 10.1371/journal.pone.0089387
    Read this paper

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Figure 1. Western blot analysis of TUBG1/2 using anti-TUBG1/2 antibody (A06313-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBG1/2 antigen affinity purified polyclonal antibody (Catalog # A06313-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUBG1/2 at approximately 51 kDa. The expected band size for TUBG1/2 is at 51 kDa.
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