GAPDH antibody [GT239]
GTX627408
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityBacteria, Canine, Drosophila, Fungus, Hamster, Human, Monkey, Mouse, Porcine, Rat, Yeast, Zebra Fish
TargetGAPDH
Overview
- SupplierGeneTex
- Product NameGAPDH antibody [GT239]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT239
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2597
- Target nameGAPDH
- Target descriptionglyceraldehyde-3-phosphate dehydrogenase
- Target synonymsG3PD, GAPD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, OCAS, p38 component, Oct1 coactivator in S phase, 38 Kd component, aging-associated gene 9 protein, epididymis secretory sperm binding protein Li 162eP, peptidyl-cysteine S-nitrosylase GAPDH
- HostMouse
- IsotypeIgG2b
- Protein IDP04406
- Protein NameGlyceraldehyde-3-phosphate dehydrogenase
- Scientific DescriptionThe product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]
- ReactivityBacteria, Canine, Drosophila, Fungus, Hamster, Human, Monkey, Mouse, Porcine, Rat, Yeast, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
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- Heo H, Park H, Lee MS, et al. TRIM22 facilitates autophagosome-lysosome fusion by mediating the association of GABARAPs and PLEKHM1. Autophagy. 2024,20(5):1098-1113. doi: 10.1080/15548627.2023.2287925Read this paper
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- Hansen FM, Kremer LS, Karayel O, et al. Mitochondrial phosphoproteomes are functionally specialized across tissues. Life Sci Alliance. 2024,7(2). doi: 10.26508/lsa.202302147Read this paper
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- Power AS, Asamudo EU, Worthington LPI, et al. Nitric Oxide Modulates Ca(2+) Leak and Arrhythmias via S-Nitrosylation of CaMKII. Circ Res. 2023,133(12):1040-1055. doi: 10.1161/CIRCRESAHA.123.323571Read this paper
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- Zhang A, Lau NA, Wong A, et al. Concurrent Targeting of HDAC and PI3K to Overcome Phenotypic Heterogeneity of Castration-resistant and Neuroendocrine Prostate Cancers. Cancer Res Commun. 2023,3(11):2358-2374. doi: 10.1158/2767-9764.CRC-23-0250Read this paper

![ICC/IF analysis of NIH-3T3 cells using GTX03407 GAPDH antibody [GT1355]. Red : Primary antibody Blue : DAPI for nuclear staining Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX03407/GTX03407_ICCIF_1_w_23053123_726.webp)
![GAPDH antibody [GT239] (HRP) detects GAPDH antibody protein at cytoplasm in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. GAPDH antibody [GT239] (HRP) (GTX627408-01) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX627408-01/GTX627408-01_41268_20170817_IHC-P_R_w_23061202_629.webp)
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637966/GTX637966_T-44886_20221209_WB_M_R_23010400_421.webp)

![WB analysis of HeLa (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate using GTX83245 GAPDH antibody [1A10].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83245/GTX83245_20170912_WB_w_23061322_915.webp)
