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GAPDH antibody [GT239]

GTX627408
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
TargetGAPDH
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Overview

  • Supplier
    GeneTex
  • Product Name
    GAPDH antibody [GT239]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT239
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2597
  • Target name
    GAPDH
  • Target description
    glyceraldehyde-3-phosphate dehydrogenase
  • Target synonyms
    G3PD, GAPD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, OCAS, p38 component, Oct1 coactivator in S phase, 38 Kd component, aging-associated gene 9 protein, epididymis secretory sperm binding protein Li 162eP, peptidyl-cysteine S-nitrosylase GAPDH
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP04406
  • Protein Name
    Glyceraldehyde-3-phosphate dehydrogenase
  • Scientific Description
    The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Jahin I, Phillips T, Marcotti S, et al. Extracellular matrix stiffness activates mechanosensitive signals but limits breast cancer cell spheroid proliferation and invasion. Front Cell Dev Biol. 2023,11:1292775. doi: 10.3389/fcell.2023.1292775
    Read this paper
  • Binh DHN, Cheng TC, Tu SH, et al. StAR-related lipid transfer domain protein 3 (STARD3) regulates HER2 and promotes HER2-positive breast cancer progression through interaction with HSP90 and SRC signaling. Am J Cancer Res. 2023,13(11):5151-5173.
    Read this paper
  • St-Denis-Bissonnette F, Cummings SE, Qiu S, et al. A clinically relevant large-scale biomanufacturing workflow to produce natural killer cells and natural killer cell-derived extracellular vesicles for cancer immunotherapy. J Extracell Vesicles. 2023,12(12):e12387. doi: 10.1002/jev2.12387
    Read this paper
  • Heo H, Park H, Lee MS, et al. TRIM22 facilitates autophagosome-lysosome fusion by mediating the association of GABARAPs and PLEKHM1. Autophagy. 2024,20(5):1098-1113. doi: 10.1080/15548627.2023.2287925
    Read this paper
  • Schmidt S, Stautner C, Vu DT, et al. A reversible state of hypometabolism in a human cellular model of sporadic Parkinson's disease. Nat Commun. 2023,14(1):7674. doi: 10.1038/s41467-023-42862-7
    Read this paper
  • Hansen FM, Kremer LS, Karayel O, et al. Mitochondrial phosphoproteomes are functionally specialized across tissues. Life Sci Alliance. 2024,7(2). doi: 10.26508/lsa.202302147
    Read this paper
  • Hu CM, Huang CC, Hsu MF, et al. Oncogenic KRAS, Mucin 4, and Activin A-Mediated Fibroblast Activation Cooperate for PanIN Initiation. Adv Sci (Weinh). 2023,10(36):e2301240. doi: 10.1002/advs.202301240
    Read this paper
  • Power AS, Asamudo EU, Worthington LPI, et al. Nitric Oxide Modulates Ca(2+) Leak and Arrhythmias via S-Nitrosylation of CaMKII. Circ Res. 2023,133(12):1040-1055. doi: 10.1161/CIRCRESAHA.123.323571
    Read this paper
  • Garcia JD, Wolfe SE, Stewart AR, et al. Distinct mechanisms drive sequential internalization and degradation of GABA(A)Rs during global ischemia and reperfusion injury. iScience. 2023,26(10):108061. doi: 10.1016/j.isci.2023.108061
    Read this paper
  • Zhang A, Lau NA, Wong A, et al. Concurrent Targeting of HDAC and PI3K to Overcome Phenotypic Heterogeneity of Castration-resistant and Neuroendocrine Prostate Cancers. Cancer Res Commun. 2023,3(11):2358-2374. doi: 10.1158/2767-9764.CRC-23-0250
    Read this paper

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Figure 1. Western blot analysis of GAPDH using anti-GAPDH antibody (A00227-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human CCRF-CEM whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAPDH antigen affinity purified polyclonal antibody (Catalog # A00227-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.
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