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ProductsBack/GAPDH antibody [GT239] (HRP)

GAPDH antibody [GT239] (HRP)

GTX627408-01
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
TargetGAPDH
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Overview

  • Supplier
    GeneTex
  • Product Name
    GAPDH antibody [GT239] (HRP)
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:20000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT239
  • Concentration
    0.62 mg/ml
  • Conjugate
    HRP
  • Gene ID2597
  • Target name
    GAPDH
  • Target description
    glyceraldehyde-3-phosphate dehydrogenase
  • Target synonyms
    G3PD, GAPD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, OCAS, p38 component, Oct1 coactivator in S phase, 38 Kd component, aging-associated gene 9 protein, epididymis secretory sperm binding protein Li 162eP, peptidyl-cysteine S-nitrosylase GAPDH
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP04406
  • Protein Name
    Glyceraldehyde-3-phosphate dehydrogenase
  • Scientific Description
    The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]
  • Reactivity
    Hamster, Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Zengel J, Wang YX, Seo JW, et al. Hardwiring tissue-specific AAV transduction in mice through engineered receptor expression. Nat Methods. 2023,20(7):1070-1081. doi: 10.1038/s41592-023-01896-x
    Read this paper
  • Escalante-Covarrubias Q, Mendoza-Viveros L, González-Suárez M, et al. Time-of-day defines NAD(+) efficacy to treat diet-induced metabolic disease by synchronizing the hepatic clock in mice. Nat Commun. 2023,14(1):1685. doi: 10.1038/s41467-023-37286-2
    Read this paper
  • Ferdous S, Shelton DA, Getz TE, et al. Deletion of histone demethylase Lsd1 (Kdm1a) during retinal development leads to defects in retinal function and structure. Front Cell Neurosci. 2023,17:1104592. doi: 10.3389/fncel.2023.1104592
    Read this paper
  • Cheng H, Zhang Y, Guo X, et al. Study on Neuroprotective Mechanism of Houshiheisan in Ischemic Stroke Based on Transcriptomics and Experimental Verification. Evid Based Complement Alternat Med. 2023,2023:8673136. doi: 10.1155/2023/8673136
    Read this paper
  • Shilts J, Crozier TWM, Teixeira-Silva A, et al. LRRC15 mediates an accessory interaction with the SARS-CoV-2 spike protein. PLoS Biol. 2023,21(2):e3001959. doi: 10.1371/journal.pbio.3001959
    Read this paper
  • Despic V, Jaffrey SR. mRNA ageing shapes the Cap2 methylome in mammalian mRNA. Nature. 2023,614(7947):358-366. doi: 10.1038/s41586-022-05668-z
    Read this paper
  • Quiles JM, Najor RH, Gonzalez E, et al. Deciphering functional roles and interplay between Beclin1 and Beclin2 in autophagosome formation and mitophagy. Sci Signal. 2023,16(770):eabo4457. doi: 10.1126/scisignal.abo4457
    Read this paper
  • Chien HM, He RY, Lee CC, et al. Nanoscopic investigation of C9orf72 poly-GA oligomers on nuclear membrane disruption by a photoinducible platform. Commun Chem. 2021,4(1):111. doi: 10.1038/s42004-021-00547-6
    Read this paper
  • Arimoto KI, Miyauchi S, Troutman TD, et al. Expansion of interferon inducible gene pool via USP18 inhibition promotes cancer cell pyroptosis. Nat Commun. 2023,14(1):251. doi: 10.1038/s41467-022-35348-5
    Read this paper
  • Sych K, Nold SP, Pfeilschifter J, et al. Expression of PKM2 in wound keratinocytes is coupled to angiogenesis during skin repair in vivo and in HaCaT keratinocytes in vitro. J Mol Med (Berl). 2023,101(1-2):151-169. doi: 10.1007/s00109-022-02280-6
    Read this paper

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Figure 1. Western blot analysis of GAPDH using anti-GAPDH antibody (A00227-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human CCRF-CEM whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAPDH antigen affinity purified polyclonal antibody (Catalog # A00227-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.
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