
ICC/IF analysis of PFA fixed HeLa cells using GTX89409 GAPDH antibody, Internal. Green : Primary antibody Blue : DAPI Negative control : Unimmunized goat IgG Permeabilization : 0.15% Triton Dilution : 5microg/ml
GAPDH antibody, Internal
GTX89409
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGAPDH
Overview
- SupplierGeneTex
- Product NameGAPDH antibody, Internal
- Delivery Days Customer9
- Antibody SpecificityThis antibody is expected to recognize both reported isoforms (NP_002037.2; NP_001243728.1).
- Application Supplier NoteWB: 0.03-0.1microg/ml. ICC/IF: 5microg/ml. IHC-P: 2microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.50 mg/ml
- ConjugateUnconjugated
- Gene ID2597
- Target nameGAPDH
- Target descriptionglyceraldehyde-3-phosphate dehydrogenase
- Target synonymsaging-associated gene 9 protein; epididymis secretory sperm binding protein Li 162eP; G3PD; GAPD; glyceraldehyde-3-phosphate dehydrogenase; HEL-S-162eP; OCAS, p38 component; Oct1 coactivator in S phase, 38 Kd component; peptidyl-cysteine S-nitrosylase GAPDH
- HostGoat
- IsotypeIgG
- Protein IDP04406
- Protein NameGlyceraldehyde-3-phosphate dehydrogenase
- Scientific DescriptionThis gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203