![Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [GT249] (GTX635705) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [GT249] (GTX635705) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635705/GTX635705_44004_20200717_WB_w_23061202_283.webp)
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [GT249] (GTX635705) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Gli1 antibody [GT249]
GTX635705
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetGLI1
Overview
- SupplierGeneTex
- Product NameGli1 antibody [GT249]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT249
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2735
- Target nameGLI1
- Target descriptionGLI family zinc finger 1
- Target synonymsGLI, PAPA8, PPD1, zinc finger protein GLI1, GLI-Kruppel family member GLI1, glioma-associated oncogene 1, glioma-associated oncogene homolog 1 (zinc finger protein), oncogene GLI
- HostMouse
- IsotypeIgG2a
- Protein IDP08151
- Protein NameZinc finger protein GLI1
- Scientific DescriptionThis gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![Gli1 antibody [GT249] detects Gli1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Gli1 stained by Gli1 antibody [GT249] (GTX635705) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Gli1 antibody [GT249] detects Gli1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Gli1 stained by Gli1 antibody [GT249] (GTX635705) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX635705/GTX635705_44004_20200814_IHC-P_M_w_23061202_989.webp)









