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WB analysis of various samples using GTX81012 GLS antibody, C-term. Lane 1: human brain lysate Lane 2: human kidney lysate Lane 3: mouse brain lysate Loading : 20 microg per lane Dilution : 1:2000
WB analysis of various samples using GTX81012 GLS antibody, C-term. Lane 1: human brain lysate Lane 2: human kidney lysate Lane 3: mouse brain lysate Loading : 20 microg per lane Dilution : 1:2000
WB analysis of various samples using GTX81012 GLS antibody, C-term. Lane 1: human brain lysate Lane 2: human kidney lysate Lane 3: mouse brain lysate Loading : 20 microg per lane Dilution : 1:2000

Glutaminase antibody, C-term

GTX81012
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGLS
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Overview

  • Supplier
    GeneTex
  • Product Name
    Glutaminase antibody, C-term
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:1000. ICC/IF: 1:25. IHC-P: 1:25. FACS: 1:25. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2744
  • Target name
    GLS
  • Target description
    glutaminase
  • Target synonyms
    AAD20, CASGID, DEE71, EIEE71, GAC, GAM, GDPAG, GLS1, KGA, glutaminase kidney isoform, mitochondrial, K-glutaminase, L-glutamine amidohydrolase, glutaminase C, glutaminase, phosphate-activated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO94925
  • Protein Name
    Glutaminase kidney isoform, mitochondrial
  • Scientific Description
    This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Lee K, Thompson EA, Gharaie S, et al. T cell metabolic reprogramming in acute kidney injury and protection by glutamine blockade. JCI Insight. 2023,8(12). doi: 10.1172/jci.insight.160345
    Read this paper
  • Potier B, Lallemant L, Parrot S, et al. DM1 Transgenic Mice Exhibit Abnormal Neurotransmitter Homeostasis and Synaptic Plasticity in Association with RNA Foci and Mis-Splicing in the Hippocampus. Int J Mol Sci. 2022,23(2). doi: 10.3390/ijms23020592
    Read this paper
  • Bruntz RC, Belshoff AC, Zhang Y, et al. Inhibition of Anaplerotic Glutaminolysis Underlies Selenite Toxicity in Human Lung Cancer. Proteomics. 2019,19(21-22):e1800486. doi: 10.1002/pmic.201800486
    Read this paper
  • Johnson MO, Wolf MM, Madden MZ, et al. Distinct Regulation of Th17 and Th1 Cell Differentiation by Glutaminase-Dependent Metabolism. Cell. 2018,175(7):1780-1795.e19. doi: 10.1016/j.cell.2018.10.001
    Read this paper
  • Xiang Y, Stine ZE, Xia J, et al. Targeted inhibition of tumor-specific glutaminase diminishes cell-autonomous tumorigenesis. J Clin Invest. 2015,125(6):2293-306. doi: 10.1172/JCI75836
    Read this paper

Datasheet

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Figure 1. Western blot analysis of Glutaminase/GLS using anti-Glutaminase/GLS antibody (A01272-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U87 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: monkey kidney tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: rat heart tissue lysates. Lane 8: rat skeletal muscle tissue lysates. Lane 9: mouse brain tissue lysates. Lane 10: mouse kidney tissue lysates. Lane 11: mouse heart tissue lysates. Lane 12: mouse skeletal muscle tissue lysates. Lane 13: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glutaminase/GLS antigen affinity purified polyclonal antibody (Catalog # A01272-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glutaminase/GLS at approximately 65-73KD. The expected band size for Glutaminase/GLS is at 73KD.
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