More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Glutaminase C (GAC) antibody
Glutaminase C (GAC) antibody validation by shRNA knock-down. Non-transfected (-) and GLS1(GAC isoform specific) shRNA-transfected MDA-MB-231 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase C (GAC) antibody (GTX131263) at a dilution of 1:5000.
Glutaminase C (GAC) antibody validation by shRNA knock-down. Non-transfected (-) and GLS1(GAC isoform specific) shRNA-transfected MDA-MB-231 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase C (GAC) antibody (GTX131263) at a dilution of 1:5000.
Glutaminase C (GAC) antibody validation by shRNA knock-down. Non-transfected (-) and GLS1(GAC isoform specific) shRNA-transfected MDA-MB-231 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase C (GAC) antibody (GTX131263) at a dilution of 1:5000.

Glutaminase C (GAC) antibody

GTX131263
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetGLS
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    Glutaminase C (GAC) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2744
  • Target name
    GLS
  • Target description
    glutaminase
  • Target synonyms
    AAD20, CASGID, DEE71, EIEE71, GAC, GAM, GDPAG, GLS1, KGA, glutaminase kidney isoform, mitochondrial, K-glutaminase, L-glutamine amidohydrolase, glutaminase C, glutaminase, phosphate-activated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO94925
  • Protein Name
    Glutaminase kidney isoform, mitochondrial
  • Scientific Description
    Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Barreca F, Aventaggiato M, Vitiello L, et al. SIRT5 Activation and Inorganic Phosphate Binding Reduce Cancer Cell Vitality by Modulating Autophagy/Mitophagy and ROS. Antioxidants (Basel). 2023,12(8). doi: 10.3390/antiox12081635
    Read this paper
  • Fazal S, Danzi MC, van Kuilenburg ABP, et al. Repeat expansions nested within tandem CNVs: a unique structural change in GLS exemplifies the diagnostic challenges of non-coding pathogenic variation. Hum Mol Genet. 2023,32(1):46-54. doi: 10.1093/hmg/ddac173
    Read this paper
  • Saladini S, Aventaggiato M, Barreca F, et al. Metformin Impairs Glutamine Metabolism and Autophagy in Tumour Cells. Cells. 2019,8(1). doi: 10.3390/cells8010049
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
RayBiotech
Anti-GLS Antibody144-03885
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetGLS
  • SizePrice
Product group Antibodies
Biosynth
GLS antibody70R-5477
  • SizePrice
Product group Antibodies
Abbexa
Glutaminase (GLS) AntibodyABX303988
ApplicationsELISA
  • SizePrice
Figure 1. Western blot analysis of Glutaminase/GLS using anti-Glutaminase/GLS antibody (A01272-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U87 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: monkey kidney tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: rat heart tissue lysates. Lane 8: rat skeletal muscle tissue lysates. Lane 9: mouse brain tissue lysates. Lane 10: mouse kidney tissue lysates. Lane 11: mouse heart tissue lysates. Lane 12: mouse skeletal muscle tissue lysates. Lane 13: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glutaminase/GLS antigen affinity purified polyclonal antibody (Catalog # A01272-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glutaminase/GLS at approximately 65-73KD. The expected band size for Glutaminase/GLS is at 73KD.
Product group Antibodies
Boster Bio
Anti-Glutaminase/GLS Antibody Picoband(r)A01272-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetGLS
  • SizePrice
Glutaminase C antibody detects Glutaminase C protein at mitochondria by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glutaminase C protein stained by Glutaminase C antibody (GTX132402) diluted at 1:500. Red: Histone H3K9ac (acetyl Lys9), a nucleus marker, stained by Histone H3K9ac (acetyl Lys9) antibody [GT464] (GTX630554) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Product group Antibodies
GeneTex
Glutaminase C (GAC) antibodyGTX132402
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetGLS
  • SizePrice
Glutaminase antibody [C1C3] detects Glutaminase protein by Western blot analysis. A. 20 μg Raw264 whole cell lysate/extract Glutaminase antibody [C1C3] (GTX114982) dilution: 1:1000
Product group Antibodies
GeneTex
Glutaminase antibody [C1C3]GTX114982
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetGLS
  • SizePrice
Glutaminase C antibody [GT1075] detects Glutaminase C protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glutaminase C protein stained by Glutaminase C antibody [GT1075] (GTX632265) diluted at 1:400. Blue: Hoechst 33342 staining.
Product group Antibodies
GeneTex
Glutaminase C (GAC) antibody [GT1075]GTX632265
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetGLS
  • SizePrice
Glutaminase C (GAC) antibody [GT3211] detects Glutaminase C (GAC) protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase C (GAC) antibody [GT3211] (GTX632317) diluted at a dilution of 1:1000.
Product group Antibodies
GeneTex
Glutaminase C (GAC) antibody [GT3211]GTX632317
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetGLS
  • SizePrice
IHC-P analysis of human brain tissue using GTX81012 GLS antibody, C-term. Antigen retrieval : heat-induced antigen retrieval with a citrate buffer (pH6) Dilution : 1:25
Product group Antibodies
References
GeneTex
Glutaminase antibody, C-termGTX81012
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetGLS
  • SizePrice
Product group Antibodies
Biomatik
GLS Polyclonal AntibodyCAC13047
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
TargetGLS
  • SizePrice