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ProductsBack/Goat anti-NQO1 (isoform a)

Goat anti-NQO1 (isoform a)

EB10772
Everest Biotech
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNQO1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-NQO1 (isoform a) Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 5microg/ml.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IF, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID1728
  • Target name
    NQO1
  • Target description
    NAD(P)H quinone dehydrogenase 1
  • Target synonyms
    azoreductase; DHQU; DIA4; diaphorase (NADH/NADPH) (cytochrome b-5 reductase); diaphorase-4; dioxin-inducible 1; DTD; DT-diaphorase; menadione reductase; NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase, quinone 1; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:quinone oxireductase; NMOR1; NMORI; phylloquinone reductase; QR1; quinone reductase 1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000894.1. Purification: Antigen affinity purified. Names and symbols: NQO1; NAD(P)H dehydrogenase, quinone 1; DHQU; DIA4; DTD; NMOR1; NMORI; QR1; DT-diaphorase; NAD(P)H dehydrogenase [quinone] 1; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:qui
  • Reactivity Supplier
    Human, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NQO1 using anti-NQO1 antibody (A00494-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NQO1 antigen affinity purified polyclonal antibody (Catalog # A00494-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NQO1 at approximately 31 kDa. The expected band size for NQO1 is at 31 kDa.
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