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ProductsBack/HDAC4 (phospho Ser246) + HDAC5 (phospho Ser259) + HDAC9 (phospho Ser220) antibody
IHC-P analysis of human breast carcinoma tissue using GTX50871 HDAC4 (phospho Ser246) + HDAC5 (phospho Ser259) + HDAC9 (phospho Ser220) antibody. Left : Primary antibody Right : Primary antibody pre-incubated with the antigen specific peptide
IHC-P analysis of human breast carcinoma tissue using GTX50871 HDAC4 (phospho Ser246) + HDAC5 (phospho Ser259) + HDAC9 (phospho Ser220) antibody. Left : Primary antibody Right : Primary antibody pre-incubated with the antigen specific peptide
IHC-P analysis of human breast carcinoma tissue using GTX50871 HDAC4 (phospho Ser246) + HDAC5 (phospho Ser259) + HDAC9 (phospho Ser220) antibody. Left : Primary antibody Right : Primary antibody pre-incubated with the antigen specific peptide

HDAC4 (phospho Ser246) + HDAC5 (phospho Ser259) + HDAC9 (phospho Ser220) antibody

GTX50871
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetHDAC9
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Overview

  • Supplier
    GeneTex
  • Product Name
    HDAC4 (phospho Ser246) + HDAC5 (phospho Ser259) + HDAC9 (phospho Ser220) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:1000. IHC-P: 1:50-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9734
  • Target name
    HDAC9
  • Target description
    histone deacetylase 9
  • Target synonyms
    ARCND4, HD7, HD7b, HD9, HDAC, HDAC7, HDAC7B, HDAC9B, HDAC9FL, HDRP, MITR, histone deacetylase 9, MEF-2 interacting transcription repressor (MITR) protein, histone deacetylase 4/5-related protein, histone deacetylase 7B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP56524
  • Protein Name
    Histone deacetylase 4
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HDAC9 using anti-HDAC9 antibody (A02177-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC9 antigen affinity purified polyclonal antibody (Catalog # A02177-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC9 at approximately 150KD. The expected band size for HDAC9 is at 150KD.
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