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ProductsBack/HIF-1 alpha (HIF1A) Mouse Monoclonal Antibody [Clone ID: 1A3]
Western blot analysis using HIF1A antibody Cat.-No AM06608SU-N against Cos7 (Lane 1), Hela (Lane 2), Jurkat (Lane 3), RAJI (Lane 4) and NIH/3T3 (Lane 5) cell lysate.
Western blot analysis using HIF1A antibody Cat.-No AM06608SU-N against Cos7 (Lane 1), Hela (Lane 2), Jurkat (Lane 3), RAJI (Lane 4) and NIH/3T3 (Lane 5) cell lysate.
Western blot analysis using HIF1A antibody Cat.-No AM06608SU-N against Cos7 (Lane 1), Hela (Lane 2), Jurkat (Lane 3), RAJI (Lane 4) and NIH/3T3 (Lane 5) cell lysate.

HIF-1 alpha (HIF1A) Mouse Monoclonal Antibody [Clone ID: 1A3]

AM06608SU-N
OriGene
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse
TargetHIF1A
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    HIF-1 alpha (HIF1A) Mouse Monoclonal Antibody [Clone ID: 1A3]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1A3
  • Gene ID3091
  • Target name
    HIF1A
  • Target description
    hypoxia inducible factor 1 subunit alpha
  • Target synonyms
    HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78, hypoxia-inducible factor 1-alpha, ARNT interacting protein, PAS domain-containing protein 8, basic-helix-loop-helix-PAS protein MOP1, class E basic helix-loop-helix protein 78, hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), hypoxia-inducible factor1alpha, member of PAS protein 1, member of PAS superfamily 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ16665
  • Protein Name
    Hypoxia-inducible factor 1-alpha
  • Scientific Description
    HIF-1 alpha (HIF1A) mouse monoclonal antibody, clone 1A3, Ascites
  • Reactivity
    Human, Monkey, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of HIF1A using anti-HIF1A antibody (A00013-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF1A antigen affinity purified polyclonal antibody (Catalog # A00013-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF1A at approximately 123KD. The expected band size for HIF1A is at 93KD.
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