WB analysis of cobalt chloride treated COS-7 nuclear extracts using GTX30105 HIF1 alpha antibody [H1alpha67]. Loading : 50microg
![ICC/IF analysis of Deferoxamine-treated HeLa cells using GTX30105 HIF1 alpha antibody [H1alpha67]. Red : primary antibody Blue : DAPI Dilution : 1 microg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX30105/GTX30105_174_ICC-IF_w_23060722_715.webp "ICC/IF analysis of Deferoxamine-treated HeLa cells using GTX30105 HIF1 alpha antibody [H1alpha67]. Red : primary antibody Blue : DAPI Dilution : 1 microg/ml")
![IHC-P analysis of human kidney tissue using GTX30105 HIF1 alpha antibody [H1alpha67].](https://www.genetex.com/upload/website/prouct_img/normal/GTX30105/GTX30105_308_IHC-P_w_23060722_320.webp "IHC-P analysis of human kidney tissue using GTX30105 HIF1 alpha antibody [H1alpha67].")
![IHC-P analysis of human glioblastoma multiforme tissue using GTX30105 HIF1 alpha antibody [H1alpha67].](https://www.genetex.com/upload/website/prouct_img/normal/GTX30105/GTX30105_532_IHC-P_w_23060722_139.webp "IHC-P analysis of human glioblastoma multiforme tissue using GTX30105 HIF1 alpha antibody [H1alpha67].")
WB analysis of cobalt chloride treated COS-7 nuclear extracts using GTX30105 HIF1 alpha antibody [H1alpha67]. Loading : 50microg
HIF1 alpha antibody [H1alpha67]
GTX30105
ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityBovine, Ferret, Human, Mammals, Monkey, Mouse, Porcine, Primate, Rabbit, Rat, Sheep, Xenopus, Yeast
TargetHIF1A
Overview
- SupplierGeneTex
- Product NameHIF1 alpha antibody [H1alpha67]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500. ICC/IF: 1:50. IHC-P: 1:20 - 1:50. IHC-Fr: 1:20 - 1:50. FACS: 1:10 - 1:1000. IP: 1:10 - 1:500. ELISA: 1:100 - 1:2000. ChIP assay: 1 - 5 microg. Gel supershift assays: 1:1 - 1:100. IHC: 1:20 - 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDH1alpha67
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3091
- Target nameHIF1A
- Target descriptionhypoxia inducible factor 1 subunit alpha
- Target synonymsHIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78, hypoxia-inducible factor 1-alpha, ARNT interacting protein, PAS domain-containing protein 8, basic-helix-loop-helix-PAS protein MOP1, class E basic helix-loop-helix protein 78, hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), hypoxia-inducible factor1alpha, member of PAS protein 1, member of PAS superfamily 1
- HostMouse
- IsotypeIgG2b
- Protein IDQ16665
- Protein NameHypoxia-inducible factor 1-alpha
- Scientific DescriptionHypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]
- ReactivityBovine, Ferret, Human, Mammals, Monkey, Mouse, Porcine, Primate, Rabbit, Rat, Sheep, Xenopus, Yeast
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Nakayama A, Roquid KA, Iring A, et al. Suppression of CCL2 angiocrine function by adrenomedullin promotes tumor growth. J Exp Med. 2023,220(1). doi: 10.1084/jem.20211628Read this paper
- García Gómez-Heras S, Largo C, Larrea JL, et al. Main histological parameters to be evaluated in an experimental model of myocardial infarct treated by stem cells on pigs. PeerJ. 2019,7:e7160. doi: 10.7717/peerj.7160Read this paper
- García-Vásquez C, Fernández-Aceñero MJ, García Gómez-Heras S, et al. Fibrin patch influences the expression of hypoxia-inducible factor-1α and nuclear factor-κBp65 factors on ischemic intestinal anastomosis. Exp Biol Med (Maywood). 2018,243(10):803-808. doi: 10.1177/1535370218777216Read this paper
- Lin CL, Hsiao CJ, Hsu CH, et al. Hypothermic neuroprotections in the brain of an echolocation bat, Hipposideros terasensis. Neuroreport. 2017,28(15):956-962. doi: 10.1097/WNR.0000000000000856Read this paper
- Mung KL, Tsui YP, Tai EW, et al. Rapid and efficient generation of neural progenitors from adult bone marrow stromal cells by hypoxic preconditioning. Stem Cell Res Ther. 2016,7(1):146.Read this paper
- Kuo HP, Lee DF, Xia W, et al. TNFalpha induces HIF-1alpha expression through activation of IKKbeta. Biochem Biophys Res Commun. 2009,389(4):640-4. doi: 10.1016/j.bbrc.2009.09.042Read this paper
- Nonomura Y, Mizoguchi F, Suzuki A, et al. Hypoxia-induced abrogation of contact-dependent inhibition of rheumatoid arthritis synovial fibroblast proliferation. J Rheumatol. 2009,36(4):698-705. doi: 10.3899/jrheum.080188Read this paper
- Catrina SB, Botusan IR, Rantanen A, et al. Hypoxia-inducible factor-1alpha and hypoxia-inducible factor-2alpha are expressed in kaposi sarcoma and modulated by insulin-like growth factor-I. Clin Cancer Res. 2006,12(15):4506-14.Read this paper
Datasheet
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