ProductsBack/HIF1 alpha antibody [H1alpha67]

WB analysis of cobalt chloride treated COS-7 nuclear extracts using GTX30115 HIF1 alpha antibody [H1alpha67].

HIF1 alpha antibody [H1alpha67]

GTX30115

ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

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Product group Antibodies

TargetHIF1A

Overview

Supplier
GeneTex
Product Name
HIF1 alpha antibody [H1alpha67] - Orthogonal Validated
Delivery Days Customer
9
Application Supplier Note
WB: 1:500 - 1:1000. ICC/IF: 1:10 - 1:500. IHC-P: 1:100 - 1:300. FACS: 1:10 - 1:1000. IP: 1:10. ELISA: 1:100 - 1:2000. ChIP assay: 1:10 - 1:500. Gel supershift assays: 1:1 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Applications
Gel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Certification
Research Use Only
Clonality
Monoclonal
Clone ID
H1alpha67
Concentration
1 mg/ml
Conjugate
Unconjugated
Gene ID3091
Target name
HIF1A
Target description
hypoxia inducible factor 1 subunit alpha
Target synonyms
ARNT interacting protein; basic-helix-loop-helix-PAS protein MOP1; bHLHe78; class E basic helix-loop-helix protein 78; HIF1; HIF-1A; HIF-1alpha; HIF-1-alpha; HIF1-ALPHA; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1-alpha; hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PAS domain-containing protein 8", "Class E basic helix-loop-helix protein 78", "Member of PAS protein 1; PASD8
Host
Mouse
Isotype
IgG2b
Protein IDQ16665
Protein Name
Hypoxia-inducible factor 1-alpha
Scientific Description
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]
Storage Instruction
-20°C or -80°C,2°C to 8°C
UNSPSC
12352203

References

Suppression of CCL2 angiocrine function by adrenomedullin promotes tumor growth.
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Main histological parameters to be evaluated in an experimental model of myocardial infarct treated by stem cells on pigs. Garcia Gomez-Heras S et al., 2019, PeerJ
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Fibrin patch influences the expression of hypoxia-inducible factor-1alpha and nuclear factor-kappaBp65 factors on ischemic intestinal anastomosis. Garcia-Vasquez C et al., 2018 Jun, Exp Biol Med (Maywood)
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Hypothermic neuroprotections in the brain of an echolocation bat, Hipposideros terasensis. Lin CL et al., 2017 Oct 18, Neuroreport
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Rapid and efficient generation of neural progenitors from adult bone marrow stromal cells by hypoxic preconditioning. Mung KL et al., 2016 Oct 7, Stem Cell Res Ther
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TNFalpha induces HIF-1alpha expression through activation of IKKbeta. Kuo HP et al., 2009 Nov 27, Biochem Biophys Res Commun
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Hypoxia-induced abrogation of contact-dependent inhibition of rheumatoid arthritis synovial fibroblast proliferation. Nonomura Y et al., 2009 Apr, J Rheumatol
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Hypoxia-inducible factor-1alpha and hypoxia-inducible factor-2alpha are expressed in kaposi sarcoma and modulated by insulin-like growth factor-I. Catrina SB et al., 2006 Aug 1, Clin Cancer Res
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HIF1 alpha antibody [H1alpha67]

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Overview

References

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