WB analysis of cobalt chloride treated COS-7 nuclear extracts using GTX30115 HIF1 alpha antibody [H1alpha67].
![IHC-P analysis of human kidney tissue using GTX30115 HIF1 alpha antibody [H1alpha67].](https://www.genetex.com/upload/website/prouct_img/normal/GTX30115/GTX30115_310_IHC-P_w_23060722_205.webp "IHC-P analysis of human kidney tissue using GTX30115 HIF1 alpha antibody [H1alpha67].")
WB analysis of cobalt chloride treated COS-7 nuclear extracts using GTX30115 HIF1 alpha antibody [H1alpha67].
HIF1 alpha antibody [H1alpha67]
GTX30115
ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityAvian, Bovine, Canine, Ferret, Human, Monkey, Mouse, Porcine, Primate, Rat, Sheep
TargetHIF1A
Overview
- SupplierGeneTex
- Product NameHIF1 alpha antibody [H1alpha67] - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:1000. ICC/IF: 1:10 - 1:500. IHC-P: 1:100 - 1:300. FACS: 1:10 - 1:1000. IP: 1:10. ELISA: 1:100 - 1:2000. ChIP assay: 1:10 - 1:500. Gel supershift assays: 1:1 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDH1alpha67
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3091
- Target nameHIF1A
- Target descriptionhypoxia inducible factor 1 subunit alpha
- Target synonymsARNT interacting protein; basic-helix-loop-helix-PAS protein MOP1; bHLHe78; class E basic helix-loop-helix protein 78; HIF1; HIF-1A; HIF-1alpha; HIF-1-alpha; HIF1-ALPHA; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1-alpha; hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PAS domain-containing protein 8", "Class E basic helix-loop-helix protein 78", "Member of PAS protein 1; PASD8
- HostMouse
- IsotypeIgG2b
- Protein IDQ16665
- Protein NameHypoxia-inducible factor 1-alpha
- Scientific DescriptionHypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]
- ReactivityAvian, Bovine, Canine, Ferret, Human, Monkey, Mouse, Porcine, Primate, Rat, Sheep
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Suppression of CCL2 angiocrine function by adrenomedullin promotes tumor growth.Read more
- Main histological parameters to be evaluated in an experimental model of myocardial infarct treated by stem cells on pigs. Garcia Gomez-Heras S et al., 2019, PeerJRead more
- Fibrin patch influences the expression of hypoxia-inducible factor-1alpha and nuclear factor-kappaBp65 factors on ischemic intestinal anastomosis. Garcia-Vasquez C et al., 2018 Jun, Exp Biol Med (Maywood)Read more
- Hypothermic neuroprotections in the brain of an echolocation bat, Hipposideros terasensis. Lin CL et al., 2017 Oct 18, NeuroreportRead more
- Rapid and efficient generation of neural progenitors from adult bone marrow stromal cells by hypoxic preconditioning. Mung KL et al., 2016 Oct 7, Stem Cell Res TherRead more
- TNFalpha induces HIF-1alpha expression through activation of IKKbeta. Kuo HP et al., 2009 Nov 27, Biochem Biophys Res CommunRead more
- Hypoxia-induced abrogation of contact-dependent inhibition of rheumatoid arthritis synovial fibroblast proliferation. Nonomura Y et al., 2009 Apr, J RheumatolRead more
- Hypoxia-inducible factor-1alpha and hypoxia-inducible factor-2alpha are expressed in kaposi sarcoma and modulated by insulin-like growth factor-I. Catrina SB et al., 2006 Aug 1, Clin Cancer ResRead more