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HIPK2 Antibody (YA1913, Rabbit)

HY-P82168
MedChem Express
TargetHIPK2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    HIPK2 Antibody (YA1913, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID28996
  • Target name
    HIPK2
  • Target description
    homeodomain interacting protein kinase 2
  • Target synonyms
    PRO0593, homeodomain-interacting protein kinase 2, hHIPk2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    HIPK2 Antibody (YA1913) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HIPK2.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HIPK2 using anti-HIPK2 antibody (M01371). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HT-1080 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIPK2 antigen affinity purified monoclonal antibody (Catalog # M01371) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIPK2 at approximately 100 kDa. The expected band size for HIPK2 is at 130 kDa.
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