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ProductsBack/HIPK2 Recombinant Antibody, AbBy Fluor-555 Conjugated

HIPK2 Recombinant Antibody, AbBy Fluor-555 Conjugated

BSM-62009R-BF555
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetHIPK2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    HIPK2 Recombinant Antibody, AbBy Fluor-555 Conjugated
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Other Conjugate
  • Gene ID28996
  • Target name
    HIPK2
  • Target description
    homeodomain interacting protein kinase 2
  • Target synonyms
    hHIPk2; homeodomain-interacting protein kinase 2; PRO0593
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H2X6
  • Protein Name
    Homeodomain-interacting protein kinase 2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HIPK2 using anti-HIPK2 antibody (M01371). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HT-1080 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIPK2 antigen affinity purified monoclonal antibody (Catalog # M01371) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIPK2 at approximately 100 kDa. The expected band size for HIPK2 is at 130 kDa.
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