More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/IHC-plus(tm) HIF1A / HIF1 Alpha Antibody

IHC-plus(tm) HIF1A / HIF1 Alpha Antibody

LS-B6182
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetHIF1A
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) HIF1A / HIF1 Alpha Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Western Blot and Immunoprecipitation. Western Blot:1:500-1:1000 (ECL). ChIP: 25 ul antibody for 15 million cells. Positive control: PC12 nuclear extracts.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:50), IP (1:1000), WB (1:500 - 1:1000) Suitable for use in Western Blot and Immunoprecipitation. Western Blot:1:500-1:1000 (ECL). ChIP: 25 ul antibody for 15 million cells. Positive control: PC12 nuclear extracts.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3091
  • Target name
    HIF1A
  • Target description
    hypoxia inducible factor 1 subunit alpha
  • Target synonyms
    HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78, hypoxia-inducible factor 1-alpha, ARNT interacting protein, PAS domain-containing protein 8, basic-helix-loop-helix-PAS protein MOP1, class E basic helix-loop-helix protein 78, hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), hypoxia-inducible factor1alpha, member of PAS protein 1, member of PAS superfamily 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
RayBiotech
Anti-HIF1A Antibody144-07684
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse
TargetHIF1A
  • SizePrice
Product group Antibodies
Biosynth
HIF1A antibody10R-10693
  • SizePrice
Figure 1. Western blot analysis of HIF1A using anti-HIF1A antibody (A00013-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF1A antigen affinity purified polyclonal antibody (Catalog # A00013-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF1A at approximately 123KD. The expected band size for HIF1A is at 93KD.
Product group Antibodies
Boster Bio
Anti-HIF-1 alpha/HIF1A Antibody Picoband(r)A00013-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetHIF1A
  • SizePrice
HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
References
GeneTex
HIF1 alpha antibodyGTX127309
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Human, Mouse, Rabbit, Rat
TargetHIF1A
  • SizePrice
Product group Antibodies
Biomatik
Hif1A Polyclonal AntibodyCAC07003
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetHIF1A
  • SizePrice
HeLa cells probed with HIF-1 Alpha Polyclonal Antibody, unconjugated (bs-0737R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)
Product group Antibodies
References
Bioss
HIF-1 Alpha Polyclonal AntibodyBS-0737R
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityChicken, Human, Mouse, Rat
TargetHIF1A
  • SizePrice
Anti-HIF1A Antibody
Product group Antibodies
Antibodies.com
Anti-HIF1A AntibodyA37506
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-HIF-1-alpha RM242 at a 1:1000 dilution.
Product group Antibodies
RevMAb Biosciences
anti-HIF-1 alpha (human), Rabbit Monoclonal (RM242)REV-31-1121-00
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetHIF1A
  • SizePrice
Product group Antibodies
Ancell Corporation
anti-Hif-1 alpha (human), mAb (ANC10G3)ANC-335-020
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetHIF1A
  • SizePrice
Western Blot analysis of HELA cells using HIF-1alpha Polyclonal Antibody
Product group Antibodies
Cusabio
HIF1A AntibodyCSB-PA002906
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetHIF1A
  • SizePrice