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ProductsBack/IHC-plus(tm) Nectin-1 / PVRL1 Antibody

IHC-plus(tm) Nectin-1 / PVRL1 Antibody

LS-B13374
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNECTIN1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) Nectin-1 / PVRL1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 39kDa/50kDa/57kDa, while the observed MW by Western blot was 55kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC (1:50 - 1:200), IHC-P (1:100), WB (1:500 - 1:2000) The predicted MW is 39kDa/50kDa/57kDa, while the observed MW by Western blot was 55kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5818
  • Target name
    NECTIN1
  • Target description
    nectin cell adhesion molecule 1
  • Target synonyms
    CD111, CLPED1, ED4, HIgR, HV1S, HVEC, OFC7, PRR, PRR1, PVRL1, PVRR, PVRR1, SK-12, nectin-1, nectin-1, ectodermal dysplasia 4 (Margarita Island type), herpes simplex virus type 1 sensitivity, herpes virus entry mediator C, herpesvirus Ig-like receptor, nectin 1, poliovirus receptor-like 1, poliovirus receptor-related 1 (herpesvirus entry mediator C), poliovirus receptor-related protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of PVRL1/NECTIN1 using anti-PVRL1/NECTIN1 antibody (A04988-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hek293 whole cell lysates, Lane 2: human CCRF-CEM whole cell lysates, Lane 3: human Sw620 whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: human SGC-7901 whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse spleen tissue lysates, Lane 9: mouse NIH/3T3 whole cell lysates, Lane 10: mouse Raw264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PVRL1/NECTIN1 antigen affinity purified polyclonal antibody (Catalog # A04988-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PVRL1/NECTIN1 at approximately 110KD. The expected band size for PVRL1/NECTIN1 is at 110KD.
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