IHC-plus(tm) SFRP1 Antibody

LifeSpan BioSciences

IHC-plus(tm) SFRP1 Antibody

23

A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. Expect cross reactivity with SFRP1 from mouse, rat and bovine sources as only a single amino acid residue change is found withi

Immunohistochemistry: LS-B57 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B57 was determined to be 20 ug/ml.. ELISA (1:5000 - 1:15000), IF (5 µg/ml), IHC, IHC-P (20 µg/ml), WB (1:200 - 1:2000) Immunohistochemistry: LS-B57 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B57 was determined to be 20 ug/ml.

1 mg/ml

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SFRP1

secreted frizzled related protein 1

frizzled-related protein; FRP; FRP1; FRP-1; FrzA; SARP2; SARP-2; secreted apoptosis-related protein 2; secreted frizzled-related protein 1; sFRP-1

-20°C,2°C to 8°C

12352203