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ProductsBack/IHC-plus(tm) SPHK / SPHK1 Antibody (N-Terminus)

IHC-plus(tm) SPHK / SPHK1 Antibody (N-Terminus)

LS-A3104
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey
TargetSPHK1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) SPHK / SPHK1 Antibody (N-Terminus)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Antigen retrieval requires Proteinase K treatment.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (10 µg/ml) Antigen retrieval requires Proteinase K treatment.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8877
  • Target name
    SPHK1
  • Target description
    sphingosine kinase 1
  • Target synonyms
    SPHK, sphingosine kinase 1, SK 1, SPK 1, acetyltransferase SPHK1
  • Host
    Rabbit
  • Reactivity
    Human, Monkey
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SPHK1 using anti-SPHK1 antibody (A01390-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human U20S whole cell lysates, Lane 7: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPHK1 antigen affinity purified polyclonal antibody (Catalog # A01390-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPHK1 at approximately 45-50 kDa. The expected band size for SPHK1 is at 45-50 kDa.
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