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Western Blot Positive WB detected in: Mouse skeletal muscle tissue All lanes: SPHK1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43, 52, 44 kDa Observed band size: 43 kDa
Western Blot Positive WB detected in: Mouse skeletal muscle tissue All lanes: SPHK1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43, 52, 44 kDa Observed band size: 43 kDa
Western Blot Positive WB detected in: Mouse skeletal muscle tissue All lanes: SPHK1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43, 52, 44 kDa Observed band size: 43 kDa

SPHK1 Antibody

CSB-PA022564LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSPHK1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SPHK1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8877
  • Target name
    SPHK1
  • Target description
    sphingosine kinase 1
  • Target synonyms
    SPHK, sphingosine kinase 1, SK 1, SPK 1, acetyltransferase SPHK1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NYA1
  • Protein Name
    Sphingosine kinase 1
  • Scientific Description
    Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SPHK1 using anti-SPHK1 antibody (A01390-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human U20S whole cell lysates, Lane 7: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPHK1 antigen affinity purified polyclonal antibody (Catalog # A01390-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPHK1 at approximately 45-50 kDa. The expected band size for SPHK1 is at 45-50 kDa.
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