More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/SPHK / SPHK1 Antibody

SPHK / SPHK1 Antibody

LS-C746745
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetSPHK1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SPHK / SPHK1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 42kDa/43kDa/51kDa, while the observed MW by Western blot was 60kDa.
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 42kDa/43kDa/51kDa, while the observed MW by Western blot was 60kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8877
  • Target name
    SPHK1
  • Target description
    sphingosine kinase 1
  • Target synonyms
    SPHK, sphingosine kinase 1, SK 1, SPK 1, acetyltransferase SPHK1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Mouse heart lysates probed with SPHK1 Polyclonal Antibody, unconjugated (bs-2652R) at 1:30 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.\n
Product group Antibodies
References
Bioss
SPHK1 Polyclonal AntibodyBS-2652R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetSPHK1
  • SizePrice
Product group Antibodies
Assay Genie
SPHK1 AntibodyCAB0139
TargetSPHK1
  • SizePrice
Product group Antibodies
Biomatik
SPHK1 Polyclonal AntibodyCAC14602
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetSPHK1
  • SizePrice
Product group Antibodies
Biosynth
SPHK1 antibody20R-SR024
  • SizePrice
Anti-SPHK1 Antibody
Product group Antibodies
Antibodies.com
Anti-SPHK1 AntibodyA83416
ApplicationsImmunoPrecipitation, Western Blot, ELISA
  • SizePrice
Figure 1. Western blot analysis of SPHK1 using anti-SPHK1 antibody (A01390-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human U20S whole cell lysates, Lane 7: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPHK1 antigen affinity purified polyclonal antibody (Catalog # A01390-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPHK1 at approximately 45-50 kDa. The expected band size for SPHK1 is at 45-50 kDa.
Product group Antibodies
Boster Bio
Anti-SPHK1 Antibody Picoband(r)A01390-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetSPHK1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-SPHK1 Antibody144-00139
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetSPHK1
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-SPHK1EB08409
ApplicationsImmunoPrecipitation, Western Blot, ELISA
TargetSPHK1
  • SizePrice
Product group Antibodies
GeneTex
SPHK1 antibody [C3], C-termGTX104724
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetSPHK1
  • SizePrice
Product group Antibodies
MedChem Express
SPHK1 Antibody (YA063, Rabbit)HY-P80337
TargetSPHK1
  • SizePrice