WB analysis of Jurkat cells stimulated with TNF-alpha (Lane 1-4, 6) or untreated (Lane 5) using GTX17943 IKK alpha (phospho Ser176/Ser180) antibody. The primary antibody was prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphoserine containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4) Dilution : 1:500
WB analysis of Jurkat cells stimulated with TNF-alpha (Lane 1-4, 6) or untreated (Lane 5) using GTX17943 IKK alpha (phospho Ser176/Ser180) antibody. The primary antibody was prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphoserine containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4) Dilution : 1:500
IKK alpha (phospho Ser176/Ser180) antibody
GTX17943
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetCHUK
Overview
- SupplierGeneTex
- Product NameIKK alpha (phospho Ser176/Ser180) antibody - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:250. ICC/IF: 1:250. FACS: 1:20. ChIP assay: 10microl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- FormulationLiquid
- Gene ID1147
- Target nameCHUK
- Target descriptioncomponent of inhibitor of nuclear factor kappa B kinase complex
- Target synonymsBPS2; conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; I-kappa-B kinase-alpha; IkB kinase alpha subunit; IKBKA; IKK1; IKKA; IKK-a kinase; IKK-alpha; inhibitor of nuclear factor kappa-B kinase subunit alpha; NFKBIKA; Nuclear factor NFkappaB inhibitor kinase alpha; TCF16; TCF-16; transcription factor 16
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- Protein IDO15111
- Protein NameInhibitor of nuclear factor kappa-B kinase subunit alpha
- Scientific DescriptionThis gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203