IL-2 Antibody [5B9-2-1]

Biorbyt

IL-2 Antibody [5B9-2-1]

10

ELISA:Sandwich:In a sandwich ELISA (assuming 100microL/well), a concentration of 4.0-8.0 microg/mL of this antibody will detect at least 32 pg/mL of recombinant murine IL-2 when used with BIorbyts Polyclonal biotinylated antigen affinity purified anti-murine IL-2 (XP-5183Bt) as the detection antibody at a concentration of at least 1.0-2.0 microg/mL. Western Blot:To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 microg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.0-2.0 ng/lane, under reducing conditions and 0.2-0.4 ng/lane, under non-reducing conditions.Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of mIL-2 (1.0 ng/mL), a concentration of 10.0-14.0 microg/mL of this antibody is required.

Western Blot, ELISA

ELISA, NeA, WB

Research Use Only

Monoclonal

5B9-2-1

Unconjugated

IL2

interleukin 2

aldesleukin; IL-2; interleukin-2; involved in regulation of T-cell clonal expansion; lymphokine; T cell growth factor; TCGF

Rat

Interleukin-2

IL-2 Antibody [5B9-2-1]

Mouse

12352203