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ProductsBack/IL3RA Mouse Monoclonal Antibody [Clone ID: 107D2.08]
IHC staining of Bouin Paraffin section of human invasive breast tumor with 107D2.08 (DAB brown)
IHC staining of Bouin Paraffin section of human invasive breast tumor with 107D2.08 (DAB brown)
IHC staining of Bouin Paraffin section of human invasive breast tumor with 107D2.08 (DAB brown)

IL3RA Mouse Monoclonal Antibody [Clone ID: 107D2.08]

DDX0300A546-100
OriGene
ApplicationsImmunoFluorescence
Product group Antibodies
TargetIL3RA
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    IL3RA Mouse Monoclonal Antibody [Clone ID: 107D2.08]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    107D2.08
  • Gene ID3563
  • Target name
    IL3RA
  • Target description
    interleukin 3 receptor subunit alpha
  • Target synonyms
    CD123, IL-3R-alpha, IL3R, IL3RAY, IL3RX, IL3RY, hIL-3Ra, interleukin-3 receptor subunit alpha, CD123 antigen, IL-3 receptor subunit alpha, IL-3R subunit alpha, interleukin 3 receptor, alpha (low affinity)
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP26951
  • Protein Name
    Interleukin-3 receptor subunit alpha
  • Scientific Description
    IL3RA mouse monoclonal antibody, clone 107D2.08
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL3RA using anti-IL3RA antibody (A04307). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL3RA antigen affinity purified polyclonal antibody (Catalog # A04307) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL3RA at approximately 60KD. The expected band size for IL3RA is at 60KD.
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