More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/iNOS antibody
Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with iNOS antibody (GTX130182) diluted at 1:500.
Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with iNOS antibody (GTX130182) diluted at 1:500.
Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with iNOS antibody (GTX130182) diluted at 1:500.

iNOS antibody

GTX130182
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetNos2
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    iNOS antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.87 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID18126
  • Target name
    Nos2
  • Target description
    nitric oxide synthase 2, inducible
  • Target synonyms
    MAC-NOS, NOS-II, Nos-2, Nos2a, i-NOS, iNOS, nitric oxide synthase, inducible, NOS type II, inducible NO synthase, inducible NOS, inducible nitric oxide synthase, macrophage NOS, nitric oxide synthase 2, inducible, macrophage, peptidyl-cysteine S-nitrosylase NOS2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29477
  • Protein Name
    Nitric oxide synthase, inducible
  • Scientific Description
    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Sánchez-Hernández L, Montero L, Mojica-Espinosa R, et al. Impact of the GK-1 adjuvant on peritoneal macrophages gene expression and phagocytosis. Immunol Lett. 2018,201:20-30. doi: 10.1016/j.imlet.2018.10.010
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Figure 1. Western blot analysis of iNOS/Nos2 using anti-iNOS/Nos2 antibody (A00368-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7(-LPS) whole cell lysates, Lane 2: mouse RAW264.7(+LPS) whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-iNOS/Nos2 antigen affinity purified polyclonal antibody (Catalog # A00368-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for iNOS/Nos2 at approximately 130 kDa. The expected band size for iNOS/Nos2 is at 130 kDa.
Product group Antibodies
Boster Bio
Anti-iNOS/Nos2 Antibody Picoband(r)A00368-4-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityMouse
TargetNos2
  • SizePrice
Product group Antibodies
Biosynth
iNOS antibody70R-50136
  • SizePrice
Formalin-fixed and paraffin-embedded : rat brain labeled with Rabbit Anti-NOS-2/iNOS Polyclonal Antibody, Unconjugated(bs-2072R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Product group Antibodies
References
Bioss
iNOS Polyclonal AntibodyBS-2072R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityMouse, Rabbit, Rat
TargetNos2
  • SizePrice
Product group Antibodies
Biomatik
Nitric Oxide Synthase 2, Inducible (NOS2) Polyclonal AntibodyCAU26993
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityMouse
TargetNos2
  • SizePrice
Product group Antibodies
Enzo Life Sciences
iNOS polyclonal antibodyENZ-ABS941-0100
  • SizePrice
Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with iNOS antibody (GTX127952) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
iNOS antibodyGTX127952
ApplicationsWestern Blot
ReactivityMouse
TargetNos2
  • SizePrice
iNOS antibody detects iNOS protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. iNOS stained by iNOS antibody (GTX130246) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Product group Antibodies
References
GeneTex
iNOS antibodyGTX130246
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityMouse, Rat
TargetNos2
  • SizePrice
Product group Antibodies
GeneTex
iNOS antibodyGTX03367
ApplicationsImmunoPrecipitation, Western Blot
ReactivityBovine, Mouse, Rat
TargetNos2
  • SizePrice
iNOS antibody [HL1213] detects iNOS protein by immunofluorescent analysis. Sample: DIV9 rat cortical neuron and Glia cell cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: iNOS stained by iNOS antibody [HL1213] (GTX636531) diluted at 1:2500. Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
Product group Antibodies
GeneTex
iNOS antibody [HL1213]GTX636531
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityMouse, Rat
TargetNos2
  • SizePrice
Detection of mouse inducible nitric oxide synthase by immunoblotting. Sample: Mouse macrophage RAW 264.7 cell line induced with IFNγ and LPS. Primary antibody: Anti-Nitric Oxide Synthase (GTX74171)
Product group Antibodies
References
GeneTex
iNOS antibodyGTX74171
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityMouse, Rat
TargetNos2
  • SizePrice