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Immunofluorescence analysis of U2OS cells using JADE1 Polyclonal Antibody.Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using JADE1 Polyclonal Antibody.Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using JADE1 Polyclonal Antibody.Blue: DAPI for nuclear staining.

JADE1 Polyclonal Antibody

E-AB-64256
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    JADE1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    Scaffold subunit of some HBO1 complexes, which have a histone H4 acetyltransferase activity. Plays a key role in HBO1 complex by directing KAT7/HBO1 specificity towards histone H4 acetylation (H4K5ac, H4K8ac and H4K12ac, regulating DNA replication initiation, regulating DNA replication initiation. May also promote acetylation of nucleosomal histone H4 by KAT5. Promotes apoptosis. May act as a renal tumor suppressor. Negatively regulates canonical Wnt signaling; at least in part, cooperates with NPHP4 in this function.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PHF17/JADE1 using anti-PHF17/JADE1 antibody (A07611-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHF17/JADE1 antigen affinity purified polyclonal antibody (Catalog # A07611-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHF17/JADE1 at approximately 96 kDa. The expected band size for PHF17/JADE1 is at 96 kDa.
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