WB analysis of nuclear enriched extracts (30 microg lysate) of HEK 293 treated with Hydrogen peroxide (100um Hydrogen peroxide for 10 min) (Lane 1), HEK 293 (Lane 2), A549 (Lane 3), HEL 92.1.7 (Lane 4), PC-3 (Lane 5), and Caco-2 (Lane 6) using GTX80695 KAP1 antibody [20C1]. Dilution : 1 microg/ml
![WB analysis of gamma irradiated HeLa cells using GTX80695 KAP1 antibody [20C1].](https://www.genetex.com/upload/website/prouct_img/normal/GTX80695/GTX80695_1955_WB_w_23061322_161.webp "WB analysis of gamma irradiated HeLa cells using GTX80695 KAP1 antibody [20C1].")
![ICC/IF analysis of HeLa cells using GTX80695 KAP1 antibody [20C1]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX80695/GTX80695_901_ICC-IF_w_23061322_258.webp "ICC/IF analysis of HeLa cells using GTX80695 KAP1 antibody [20C1]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature")
![ICC/IF analysis of gamma irradiated HeLa cells using GTX80695 KAP1 antibody [20C1].](https://www.genetex.com/upload/website/prouct_img/normal/GTX80695/GTX80695_902_ICC-IF_w_23061322_811.webp "ICC/IF analysis of gamma irradiated HeLa cells using GTX80695 KAP1 antibody [20C1].")
WB analysis of nuclear enriched extracts (30 microg lysate) of HEK 293 treated with Hydrogen peroxide (100um Hydrogen peroxide for 10 min) (Lane 1), HEK 293 (Lane 2), A549 (Lane 3), HEL 92.1.7 (Lane 4), PC-3 (Lane 5), and Caco-2 (Lane 6) using GTX80695 KAP1 antibody [20C1]. Dilution : 1 microg/ml
KAP1 antibody [20C1]
GTX80695
ApplicationsGel Shift Assay, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
Product group Antibodies
TargetTRIM28
Overview
- SupplierGeneTex
- Product NameKAP1 antibody [20C1] - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1 - 3 microg/ml. ICC/IF: 2 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsGel Shift Assay, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID20C1
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID10155
- Target nameTRIM28
- Target descriptiontripartite motif containing 28
- Target synonymsE3 SUMO-protein ligase TRIM28; KAP1; KAP-1; KRAB [Kruppel-associated box domain]-associated protein 1; KRAB-interacting protein 1; KRIP-1; nuclear corepressor KAP-1; PPP1R157; protein phosphatase 1, regulatory subunit 157; RING finger protein 96; RING-type E3 ubiquitin transferase TIF1-beta; RNF96; TF1B; TIF1B; TIF1-beta; transcription intermediary factor 1-beta; transcriptional intermediary factor 1-beta
- HostMouse
- IsotypeIgG
- Protein IDQ13263
- Protein NameTranscription intermediary factor 1-beta
- Scientific DescriptionThe protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
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