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Lamin B2 Monoclonal Antibody

CAB5001
Assay Genie
TargetLMNB2
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Lamin B2 Rabbit mAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IHC,IF
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID84823
  • Target name
    LMNB2
  • Target description
    lamin B2
  • Target synonyms
    EPM9, LAMB2, LMN2, MCPH27, lamin-B2, epididymis secretory sperm binding protein, lamin B3
  • Host
    Rabbit
  • Protein IDQ03252
  • Protein Name
    Lamin-B2
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251 MG. Lane 4: Human plasma. Lane 5: Human Liver tissue. Lane 6: Human Tonsil tissue
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Figure 1. Western blot analysis of Lamin B2/LMNB2 using anti-Lamin B2/LMNB2 antibody (A05348-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lamin B2/LMNB2 antigen affinity purified polyclonal antibody (Catalog # A05348-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lamin B2/LMNB2 at approximately 72 kDa. The expected band size for Lamin B2/LMNB2 is at 68 kDa.
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