![FACS analysis of HeLa cells using GTX60594 LC3B antibody [5H12]. Green : LC3B Red : negative control FACS analysis of HeLa cells using GTX60594 LC3B antibody [5H12]. Green : LC3B Red : negative control](https://www.genetex.com/upload/website/prouct_img/normal/GTX60594/GTX60594_20170912_FACS_w_23061123_292.webp)
FACS analysis of HeLa cells using GTX60594 LC3B antibody [5H12]. Green : LC3B Red : negative control
LC3B antibody [5H12]
GTX60594
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetMAP1LC3B
Overview
- SupplierGeneTex
- Product NameLC3B antibody [5H12]
- Delivery Days Customer9
- Application Supplier NoteWB: 1/500 - 1/2000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID5H12
- ConjugateUnconjugated
- Gene ID81631
- Target nameMAP1LC3B
- Target descriptionmicrotubule associated protein 1 light chain 3 beta
- Target synonymsATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a, microtubule-associated protein 1 light chain 3 beta, MAP1 light chain 3-like protein 2, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, autophagy-related ubiquitin-like modifier LC3 B, microtubule-associated proteins 1A/1B light chain 3B
- HostMouse
- IsotypeIgG1
- Protein IDQ9GZQ8
- Protein NameMicrotubule-associated protein 1 light chain 3 beta
- Scientific DescriptionThe product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![WB analysis of human LC3B (AA: 1-125) recombinant protein using GTX60594 LC3B antibody [5H12]. WB analysis of human LC3B (AA: 1-125) recombinant protein using GTX60594 LC3B antibody [5H12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60594/GTX60594_20170912_WB_1_w_23061123_204.webp)
![ELISA analysis of antigen using GTX60594 LC3B antibody [5H12].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng ELISA analysis of antigen using GTX60594 LC3B antibody [5H12].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng](https://www.genetex.com/upload/website/prouct_img/normal/GTX60594/GTX60594_20170912_ELISA_w_23061123_873.webp)
![WB analysis of HEK293 (1) and LC3B (AA: 1-125)-hIgGFc transfected HEK293 (2) cell lysate using GTX60594 LC3B antibody [5H12]. WB analysis of HEK293 (1) and LC3B (AA: 1-125)-hIgGFc transfected HEK293 (2) cell lysate using GTX60594 LC3B antibody [5H12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60594/GTX60594_20170912_WB_w_23061123_572.webp)

![ICC/IF analysis of PFA-fixed HeLa cells with/without Chloroquine (50 μM, 37oC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187]. Right : untreated HeLa cells Left : HeLa cells with 50 μM Chloroquine (20 hrs, 37oC) treatment Orange : Primary antibody Blue : DAPI Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00949/GTX00949_20200327_ICC-IF_43_w_23053121_449.webp)
![Untreated (–) and treated (+) HeLa whole cell extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT3612] (GTX632501) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632501/GTX632501_42261_20151231_WB_chloroquine_w_23061202_146.webp)


