Untreated (–) and treated (+) HeLa whole cell extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT3612] (GTX632501) diluted at 1:500.
![Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT3612] (GTX632501) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632501/GTX632501_42261_20151105_WB_Thapsigargin_w_23061202_916.webp "Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT3612] (GTX632501) diluted at 1:500.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT3612] (GTX632501) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632501/GTX632501_42156_20151105_WB_B_w_23061202_140.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT3612] (GTX632501) diluted at 1:500.")
![LC3B antibody [GT3612] detects LC3B protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: LC3B protein stained by LC3B antibody [GT3612] (GTX632501) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632501/GTX632501_42261_20151230_IFA_w_23061202_712.webp "LC3B antibody [GT3612] detects LC3B protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: LC3B protein stained by LC3B antibody [GT3612] (GTX632501) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.")
Untreated (–) and treated (+) HeLa whole cell extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT3612] (GTX632501) diluted at 1:500.
LC3B antibody [GT3612]
GTX632501
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetMAP1LC3B
Overview
- SupplierGeneTex
- Product NameLC3B antibody [GT3612]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT3612
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID81631
- Target nameMAP1LC3B
- Target descriptionmicrotubule associated protein 1 light chain 3 beta
- Target synonymsATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a, microtubule-associated protein 1 light chain 3 beta, MAP1 light chain 3-like protein 2, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, autophagy-related ubiquitin-like modifier LC3 B, microtubule-associated proteins 1A/1B light chain 3B
- HostMouse
- IsotypeIgG2a
- Protein IDQ9GZQ8
- Protein NameMicrotubule-associated protein 1 light chain 3 beta
- Scientific DescriptionThe product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Zhang M, Yan W, Yu Y, et al. Liraglutide ameliorates diabetes-associated cognitive dysfunction via rescuing autophagic flux. J Pharmacol Sci. 2021,147(3):234-244. doi: 10.1016/j.jphs.2021.07.004Read this paper
- Wan C, Tai J, Zhang J, et al. Silver nanoparticles selectively induce human oncogenic γ-herpesvirus-related cancer cell death through reactivating viral lytic replication. Cell Death Dis. 2019,10(6):392. doi: 10.1038/s41419-019-1624-zRead this paper
- Tsai YG, Wen YS, Wang JY, et al. Complement regulatory protein CD46 induces autophagy against oxidative stress-mediated apoptosis in normal and asthmatic airway epithelium. Sci Rep. 2018,8(1):12973. doi: 10.1038/s41598-018-31317-5Read this paper
Datasheet
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