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ProductsBack/LRRC32 Rabbit pAb, FITC conjugated

LRRC32 Rabbit pAb, FITC conjugated

ORB189337
Biorbyt
ApplicationsImmunoFluorescence, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Primate
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Overview

  • Supplier
    Biorbyt
  • Product Name
    LRRC32 Rabbit pAb, FITC conjugated
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry
  • Applications Supplier
    IF=1:100-500, ICC/IF=1:100-500 ICC, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    FITC
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    LRRC32 Rabbit pAb, FITC conjugated
  • Reactivity
    Human, Primate
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LRRC32 using anti-LRRC32 antibody (A08199-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SK-OV-3 whole cell lysates, Lane 2: human PANC-1 whole cell lysates, Lane 3: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC32 antigen affinity purified polyclonal antibody (Catalog # A08199-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRRC32 at approximately 72KD. The expected band size for LRRC32 is at 72KD.
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