Boiled and unboiled various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAG antibody [HL3469] (GTX641354) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Boiled and unboiled human tissue extract were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAG antibody [HL3469] (GTX641354) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641354/GTX641354_T-45600_20241122_WB_brain_ub_24112622_110.webp "Boiled and unboiled human tissue extract were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAG antibody [HL3469] (GTX641354) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Boiled and unboiled rat tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAG antibody [HL3469] (GTX641354) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641354/GTX641354_T-45600_20241206_WB_R_brain_ub_24121100_371.webp "Boiled and unboiled rat tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAG antibody [HL3469] (GTX641354) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![MAG antibody [HL3469] detects MAG protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: MAG stained by MAG antibody [HL3469] (GTX641354) diluted at 1:250. Red: NF-H stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX641354/GTX641354_T-45600_20250117_IHC-Fr_M_25020422_861.webp "MAG antibody [HL3469] detects MAG protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: MAG stained by MAG antibody [HL3469] (GTX641354) diluted at 1:250. Red: NF-H stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![MAG antibody [HL3469] detects MAG protein by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Green: MAG stained by MAG antibody [HL3469] (GTX641354) diluted at 1:400. Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX641354/GTX641354_T-45600_20250117_IHC-P_R_25020422_562.webp "MAG antibody [HL3469] detects MAG protein by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Green: MAG stained by MAG antibody [HL3469] (GTX641354) diluted at 1:400. Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![MAG antibody [HL3469] detects MAG protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. MAG stained by MAG antibody [HL3469] (GTX641354) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Corresponding RNA levels (RPKM) in the tissues are based on NCBI database.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641354/GTX641354_T-45600_20250113_IHC-P_M_Multiple_RPKM_25020422_779.webp "MAG antibody [HL3469] detects MAG protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. MAG stained by MAG antibody [HL3469] (GTX641354) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Corresponding RNA levels (RPKM) in the tissues are based on NCBI database.")
Boiled and unboiled various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAG antibody [HL3469] (GTX641354) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
MAG antibody [HL3469]
GTX641354
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAG
Overview
- SupplierGeneTex
- Product NameMAG antibody [HL3469]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3469
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID4099
- Target nameMAG
- Target descriptionmyelin associated glycoprotein
- Target synonymsGMA, S-MAG, SIGLEC-4A, SIGLEC4, SIGLEC4A, SPG75, myelin-associated glycoprotein, sialic acid binding Ig-like lectin 4A, sialic acid-binding immunoglobulin-like lectin 4A
- HostRabbit
- IsotypeIgG
- Protein IDP20916
- Protein NameMyelin-associated glycoprotein
- Scientific DescriptionThe protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2010]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
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