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WB analysis of NIH-3T3 cell lysate using GTX00686 ATF6 antibody [37-1]. The asterisk denotes an unglycosylated form of pATF6alpha(P). ATF6alpha is constitutively expressed as pATF6alpha(P), and converted to pATF6alpha(N) ( > 50-kDa protein) in ER-stressed cells. pATF6alpha(P) : the endogenous precursor ATF6alpha (~90kDa) pATF6alpha(N) : the cleaved product of ATF6alpha(>50kDa) Lane 1 : untreated Lane 2 : 1mM DTT (reducing reagent) for 1 h Lane 3 : 2 microg/ml tunicamycin (inhibitor of N-glycosylation) for 3 h Lane 4 : 2 microg/ml tunicamycin (inhibitor of N-glycosylation) for 7 h. Loading : 50microg
Product group Antibodies
References
GeneTex
ATF6 antibody [37-1]GTX00686
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman
TargetATF6
  • SizePrice
ICC/IF analysis of HeLa cells using GTX00687 ATF6 antibody [1-7]. Green : Primary antibody Blue : DAPI Dilution : 1:100
Product group Antibodies
References
GeneTex
ATF6 antibody [1-7]GTX00687
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetATF6
  • SizePrice
ICC/IF analysis of HeLa cells using GTX00688 KDM5A / Jarid1A / RBBP2 antibody [18E8]. Dilution : 1:2000 Fixation : 4% PFA, overnight Permeabilization : 0.25% Triton X-100 in PBS for 10 min
Product group Antibodies
References
GeneTex
KDM5A / Jarid1A / RBBP2 antibody [18E8]GTX00688
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse
TargetKDM5A
  • SizePrice
ICC/IF analysis of mouse spermatozoa using GTX00689 IZUMO1 antibody [#125]. Green : Primary antibody Dilution : 4 microg/ml Fixation : ice-cold 100% ethanol
Product group Antibodies
References
GeneTex
IZUMO1 antibody [#125]GTX00689
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse
TargetIzumo1
  • SizePrice
ICC/IF analysis of fresh mouse sperms using GTX00690 ADAM1B antibody [#158]. Dilution : 1:300 Fixation : 4% PFA
Product group Antibodies
References
GeneTex
ADAM1B antibody [#158]GTX00690
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityMouse
TargetAdam1b
  • SizePrice
ICC/IF analysis of a representative time-lapse view of sperm-egg fusion using GTX00691 ADAM1B antibody [#57]. The initiation of fusion (time 0) was detected by transfer of Hoechst 33342 dye to sperm in the same area (indicated by arrowheads). The diffusion of membrane in the postacrosomal area identified by ADAM1B (green) started about 60 s after the initiation of sperm-egg fusion and was accompanied by expansion of the Hoechst 33342 staining area toward the posterior head.
Product group Antibodies
References
GeneTex
ADAM1B antibody [#57]GTX00691
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityMouse
TargetAdam1b
  • SizePrice
ICC/IF analysis of Tetrahymena themophila cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. The open arrow indicates the micronucleus. Insets are magnified images showing the position of the micronucleus. Both clone 13C2 and 21A10 stained the macronuclear periphery of T. thermophila. 13C2 mAb was highly specific to the macronucleus. In contrast, in addition to clear macronuclear staining, clone 21A10 also stained the micronuclear periphery. This indicates that 21A10 recognizes Nups localizing to the micronucleus such as Nup308 in addition to MacNup98A. Neither could 2H10 nor 414 could stain nuclear periphery of Tetrahymena. Green : Primary antibody Violet : DAPI Dilution : 0.5 microg/ml Fixation : Cold Methanol (-30 degree C) for 30 min
Product group Antibodies
References
GeneTex
NUP98 antibody [13C2]GTX00693
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Yeast, Other Species
TargetTTHERM_00071070
  • SizePrice
ICC/IF analysis of COS1 cell lysate overexpressed GFP and GST tagged ZIPK fusion protein using GTX00694 GFP antibody [1A5]. Note that fluorescence by the immunofluorescent staining using 1A5 antibody is much stronger than fluorescence of GFP. Red : Primary antibody Green : Fluorescence of GFP
Product group Antibodies
References
GeneTex
GFP antibody [1A5]GTX00694
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
ReactivityOther Species
  • SizePrice
ICC/IF analysis of Tetrahymena themophila cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. 13C2 mAb was highly specific to the macronucleus. In contrast, in addition to clear macronuclear staining, 21A10 mAb also stained the micronuclear periphery. This indicates that 21A10 mAb recognizes Nups localizing to the micronucleus such as Nup308 in addition to MacNup98A. Neither mABs 2H10 nor 414 could stain nuclear periphery of Tetrahymena. Green : Primary antibody Violet : DAPI Dilution : 0.5 microg/ml Fixation : Cold Methanol (-30 degree C) for 30 min
Product group Antibodies
References
GeneTex
NUP98 antibody [21A10]GTX00695
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Yeast, Other Species
TargetNUP98
  • SizePrice
ICC/IF analysis of HeLa cells using GTX00696 Nucleoporin p62 antibody [2A11]. Left : Nup62 protein focused on nuclear surface Right : Nup62 protein focused on nuclear rim Dilution : 1:400 Fixation : 4% PFA Permeabilization : 0.5% Triton X-100
Product group Antibodies
References
GeneTex
Nucleoporin p62 antibody [2A11]GTX00696
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Monkey
TargetNUP62
  • SizePrice
ICC/IF analysis of HeLa cells using GTX00697 NUP98 antibody [2H10]. Dilution : 0.5 microg/ml Fixation : Cold Methanol (-30 degree C) for 30 min
Product group Antibodies
References
GeneTex
NUP98 antibody [2H10]GTX00697
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat, Yeast
TargetNUP98
  • SizePrice
WB analysis of various samples using GTX00698 KPNA4 antibody [3D10]. Lane 1 : HeLa whole cell lysate Lane 2 : COS7 whole cell lysate Lane 3 : L929 whole cell lysate Lane 4 : NRK whole cell lysate Lane 5 : BHK whole cell lysate Lane 6 : MDBK whole cell lysate
Product group Antibodies
References
GeneTex
KPNA4 antibody [3D10]GTX00698
ApplicationsWestern Blot, ELISA
ReactivityBovine, Canine, Hamster, Human, Monkey, Mouse, Primate, Rat
TargetKpna4
  • SizePrice
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