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Immunohistochemical analysis of paraffin-embedded human tonsil, using CD86 Antibody (M00220-1) CD86 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD86 Antibody (M00220-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-CD86/B7 2 Rabbit Monoclonal AntibodyM00220-1
Western blot analysis of LRRK2 expression in GFP-LRRK2 lysate.
Product group Antibodies
Boster Bio
Anti-LRRK2 Rabbit Monoclonal AntibodyM00221-1
Immunohistochemical analysis of paraffin-embedded human thyroid, using LRRK2 Antibody(M00221-2) LRRK2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-LRRK2 Antibody (M00221-2)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-LRRK2 Rabbit Monoclonal AntibodyM00221-2
Western blot analysis of LRRK2 in HEK293 cell lysate transfected with 3*Flag wild type, full length LRRK2 (M00221). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRK2 monoclonal antibody (Catalog # M00221) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRRK2
Product group Antibodies
Boster Bio
Anti-LRRK2 Rabbit Monoclonal AntibodyM00221
Western blot analysis of BRG1 expression in HeLa cell lysate.
Product group Antibodies
Boster Bio
Anti-BRG1 Rabbit Monoclonal AntibodyM00223
Western blot analysis of TNFAIP3 expression in Jurkat cell treated with TNF + TPA lysate (M00224). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFAIP3 monoclonal antibody (Catalog # M00224) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNFAIP3
Product group Antibodies
Boster Bio
Anti-TNFAIP3/A20 Rabbit Monoclonal AntibodyM00224
Western blot analysis of AhR expression in HEK293 cell lysate.
Product group Antibodies
Boster Bio
Anti-AhR Rabbit Monoclonal AntibodyM00225
Immunohistochemical analysis of paraffin-embedded human bladder cancer, using GAPDH Antibody.
Product group Antibodies
Boster Bio
Anti-GAPDH Rabbit Monoclonal AntibodyM00227-1
Immunofluorescent analysis of HeLa cells, using GAPDH Antibody.
Product group Antibodies
Boster Bio
Anti-GAPDH Rabbit Monoclonal AntibodyM00227
Western blot analysis of Tuberin expression in Jurkat cell lysate.
Product group Antibodies
Boster Bio
Anti-Tuberin Rabbit Monoclonal AntibodyM00229
Western blot analysis of IL4 expression in human tonsil lysate.
Product group Antibodies
Boster Bio
Anti-IL4 Rabbit Monoclonal AntibodyM00230
Western blot analysis of FGFR2 expression in (1) MCF-7 cell lysate; (2) Mouse brain lysate (M00231). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGFR2 monoclonal antibody (Catalog # M00231) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGFR2
Product group Antibodies
Boster Bio
Anti-FGFR2 Rabbit Monoclonal AntibodyM00231
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