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Are you looking for life science and diagnostic reagents? We offer one of the most extensive ranges in the Benelux. There are currently more than 5 million products in our webshop, which are manufactured by more than 130 suppliers. This includes both life science and diagnostic reagents. We hope to support your research with everything you need.

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Western blot analysis of AMPK beta 1 expression in (1) 293T cell lysate treated with Lambda lysate; (2) 293T cell lysate (P01420-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKAA2 monoclonal antibody (Catalog # P01420-1) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKAA2
Product group Antibodies
Boster Bio
Anti-Phospho-AMPK alpha 2 (S491) PRKAA2 Rabbit Monoclonal AntibodyP01420-1
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetPRKAA2
  • SizePrice
Western blot analysis of Phospho-AMPK alpha (T172) expression in Mouse heart lysate.
Product group Antibodies
Boster Bio
Anti-Phospho-AMPK alpha (T172) PRKAA2 Rabbit Monoclonal AntibodyP01420-2
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetPRKAA1
  • SizePrice
Western blot analysis of Phospho-AMPK alpha 2 (S345) expression in (1) 293T cell lysate treated with Lambda Phosphatase; (2) 293T cell lysate (P01420). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKAA2 monoclonal antibody (Catalog # P01420) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKAA2
Product group Antibodies
Boster Bio
Anti-Phospho-AMPK alpha 2 (S345) PRKAA2 Rabbit Monoclonal AntibodyP01420
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetPRKAA2
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human pancreas, using Phospho-Smad5 (S463/465) Antibody(P01423-1) SMAD5 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD5 Antibody (P01423-1)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal AntibodyP01423-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSMAD5
  • SizePrice
Western blot analysis of Phospho-Smad5 (S463/S465) expression in (1) HeLa cell lysate; (2) HeLa cell treated with BMP-4 lysate (P01423). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD5 monoclonal antibody (Catalog # P01423) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD5
Product group Antibodies
Boster Bio
Anti-Phospho-Smad1/5/9 (S463/S465/S467) Rabbit Monoclonal AntibodyP01423
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetSMAD5
  • SizePrice
Western blot analysis of Lyn phosphorylation expression in Jurkat cell lysate treated with Pervanadate.
Product group Antibodies
Boster Bio
Anti-Phospho-Lyn (Y396) Rabbit Monoclonal AntibodyP01424-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse
TargetLYN
  • SizePrice
Anti-TNR antibody, PA1695-1, IHC(P) IHC(P): Rat Brain Tissue
Product group Antibodies
Boster Bio
Anti-Tenascin-R TNR Antibody Picoband(r)PA1695-1
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityChicken, Human, Mouse, Rat
TargetTNR
  • SizePrice
Anti-CYP11A1 antibody, PA1698-1, Western blotting All lanes: Anti CYP11A1 (PA1698-1) at 0.5ug/ml Lane 1: Rat Testis Tissue Lysate at 50ug Lane 2: Mouse Testis Tissue Lysate at 50ug Lane 3: Mouse Brain Tissue Lysate at 50ug Predicted bind size: 60KD Observed bind size: 60KD
Product group Antibodies
Boster Bio
Anti-CYP11A1 Antibody Picoband(r)PA1698-1
ApplicationsWestern Blot
ReactivityBovine, Mouse, Rat
TargetCyp11a1
  • SizePrice
Western blot analysis of HEXA using anti-HEXA antibody (PA1787-2).
Product group Antibodies
Boster Bio
Anti-HEXA Antibody Picoband(r)PA1787-2
ApplicationsWestern Blot
ReactivityHuman, Monkey
TargetHEXA
  • SizePrice
Anti-LASP1 antibody, PA1791-1, Western blotting Lane 1: HELA Cell Lysate Lane 2: MCF-7 Cell Lysate
Product group Antibodies
Boster Bio
Anti-LIM and SH3 domain protein 1 LASP1 Antibody Picoband(r)PA1791-1
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Monkey
TargetLASP1
  • SizePrice
Anti-FES antibody, PA1854-1, All Western blotting All lanes: Anti-FES(PA1854-1) at 0.5ug/ml
Product group Antibodies
Boster Bio
Anti-FES Antibody Picoband(r)PA1854-1
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetFES
  • SizePrice
Anti-CD11b antibody, PA1943-1, All Western blotting All lanes: Anti-ITGAM(PA1943-1) at 0.5ug/ml
Product group Antibodies
Boster Bio
Anti-CD11b/ITGAM Antibody Picoband(r)PA1943-1
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetITGAM
  • SizePrice
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