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IHC-P analysis of mouse liver tissue using GTX31914 ME1 antibody. Working concentration : 5 microg/ml
IHC-P analysis of mouse liver tissue using GTX31914 ME1 antibody. Working concentration : 5 microg/ml
IHC-P analysis of mouse liver tissue using GTX31914 ME1 antibody. Working concentration : 5 microg/ml

ME1 antibody

GTX31914
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetME1
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Overview

  • Supplier
    GeneTex
  • Product Name
    ME1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 - 2 microg/mL. IHC-P: 5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4199
  • Target name
    ME1
  • Target description
    malic enzyme 1
  • Target synonyms
    HUMNDME; malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)); malic enzyme 1, NADP(+)-dependent, cytosolic; malic enzyme 1, soluble; Malic enzyme, cytoplasmic; MES; NADP-dependent malic enzyme; NADP-ME; pyruvic-malic carboxylase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48163
  • Protein Name
    NADP-dependent malic enzyme
  • Scientific Description
    This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ME1 using anti-ME1 antibody (A03449-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ME1 antigen affinity purified polyclonal antibody (Catalog # A03449-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ME1 at approximately 64 kDa. The expected band size for ME1 is at 64 kDa.
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