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mH2A1 Polyclonal Antibody

RD84246A
Reddot Biotech
ApplicationsImmunoFluorescence, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMACROH2A1

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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    mH2A1 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9555
  • Target name
    MACROH2A1
  • Target description
    macroH2A.1 histone
  • Target synonyms
    H2A.y, H2A/y, H2AF12M, H2AFY, MACROH2A1.1, mH2A1, macroH2A1.2, core histone macro-H2A.1, H2A histone family member Y, histone H2A.y, histone macroH2A1, histone macroH2A1.1, histone macroH2A1.2, medulloblastoma antigen MU-MB-50.205
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of H2AFY/MACROH2A1 using anti-H2AFY/MACROH2A1 antibody (A04635-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-H2AFY/MACROH2A1 antigen affinity purified polyclonal antibody (Catalog # A04635-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for H2AFY/MACROH2A1 at approximately 39 kDa. The expected band size for H2AFY/MACROH2A1 is at 40 kDa.
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