WB analysis of HL-60 lysate using MIF antibody at a dilution of 1:1,000.
WB analysis of HL-60 lysate using MIF antibody at a dilution of 1:1,000.
MIF antibody [4E4]
GTX53741
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
TargetMIF
Overview
- SupplierGeneTex
- Product NameMIF antibody [4E4]
- Delivery Days Customer9
- Application Supplier NoteThe antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:1,000.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID4282
- Target nameMIF
- Target descriptionmacrophage migration inhibitory factor
- Target synonymsGIF, GLIF, MMIF, macrophage migration inhibitory factor, L-dopachrome isomerase, L-dopachrome tautomerase, epididymis secretory sperm binding protein, macrophage migration inhibitory factor (glycosylation-inhibiting factor), phenylpyruvate tautomerase
- HostMouse
- IsotypeIgG1
- Protein IDP14174
- Protein NameMacrophage migration inhibitory factor
- Scientific DescriptionThis gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Human embryonic stem cells secrete macrophage migration inhibitory factor: A novel finding.Read more
Datasheet
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![Western blot analysis of GeneTexs IgY fraction of Chicken-anti-Human MIF polyclonal antibody shows the detection of 100 microg of recombinant MIF present in a lysate. Similar detection of MIF will occur when human serum is analyzed. In lane 1 no reaction is observed in the control whereas lane 2 shows a single band at 12.3 kDa. A 4-20% gradient gel was used to separate the proteins by SDS-PAGE. The protein was transferred to nitro-cellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye800 conjugated Gt-a-Chicken Rabbit IgG [H&L] for 1 h at room temperature. LICORs OdysseyR Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.](https://www.genetex.com/upload/website/prouct_img/normal/GTX48478/GTX48478_20160330_WB_w_23060823_846.webp)