Mouse anti Cytokeratin 8 / Keratin K8

Nordic-MUbio

Mouse anti Cytokeratin 8 / Keratin K8

7

35betaH11 reacts exclusively with Cytokeratin 8 which is present in glandular-type epithelia and most carcinomas derived thereof. It is in general not reactive in non-epithelial tissues and cells.

35betaH11 is reactive on frozen tissue sections after fixation in methanol. It is particularly effective on paraffin-embedded tissues after microwave pretreatment of the slides. In addition, 35betaH11 is suitable for immunocytochemistry, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:500 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Immunohistochemistry (frozen);Immunohistochemistry (paraffin);Western Blotting

Primary antibodies

Research Use Only

Monoclonal

35betaH11

KRT8

keratin 8

CARD2; CK8; CK-8; CYK8; cytokeratin-8; K2C8; K8; keratin 8, type II; keratin, type II cytoskeletal 8; KO; type-II keratin Kb8

Mouse

IgM

Keratin, type II cytoskeletal 8

35betaH11 is a mouse monoclonal IgM antibody derived by fusion of NS-1 mouse myeloma cells with spleen cells from a Balb/c mouse immunized with a cytoskeletal preparation of the human hepotocellular carcinoma cell line Hep3B.

Human, Zebra Fish

Human;Zebrafish

12352203