![ICC/IF analysis of PFA-fixed U2OS cells using GTX04863 NDP52 antibody [9E2F2]. Orange : Primary antibody Blue : DAPI Antigen retrieval : IHC enzyme antigen retrieval reagent for 15 mins Dilution : 5 μg/mL ICC/IF analysis of PFA-fixed U2OS cells using GTX04863 NDP52 antibody [9E2F2]. Orange : Primary antibody Blue : DAPI Antigen retrieval : IHC enzyme antigen retrieval reagent for 15 mins Dilution : 5 μg/mL](https://www.genetex.com/upload/website/prouct_img/normal/GTX04863/GTX04863_20240531_ICCIF_24053100_480.webp)
ICC/IF analysis of PFA-fixed U2OS cells using GTX04863 NDP52 antibody [9E2F2]. Orange : Primary antibody Blue : DAPI Antigen retrieval : IHC enzyme antigen retrieval reagent for 15 mins Dilution : 5 μg/mL
NDP52 antibody [9E2F2]
GTX04863
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCALCOCO2
Overview
- SupplierGeneTex
- Product NameNDP52 antibody [9E2F2]
- Delivery Days Customer7
- Application Supplier NoteWB: 0.25-0.5 microg/ml. ICC/IF: 5 microg/ml. FCM: 1-3microg/1x106 cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID9E2F2
- Concentration0.5 mg/ml
- ConjugateUnconjugated
- Gene ID10241
- Target nameCALCOCO2
- Target descriptioncalcium binding and coiled-coil domain 2
- Target synonymsNDP52, calcium-binding and coiled-coil domain-containing protein 2, antigen nuclear dot 52 kDa protein, nuclear domain 10 protein 52, nuclear domain 10 protein NDP52, nuclear dot protein 52
- HostMouse
- IsotypeIgG2b
- Protein IDQ13137
- Protein NameCalcium-binding and coiled-coil domain-containing protein 2
- Scientific DescriptionThis gene encodes a coiled-coil domain-containing protein. The encoded protein functions as a receptor for ubiquitin-coated bacteria and plays an important role in innate immunity by mediating macroautophagy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![FACS analysis of MCF7 cells using GTX04863 NDP52 antibody [9E2F2]. Blue line : Primary antibody Green line : Isotype control Red line : Unlabelled sample Dilution : 1μg/1x10? cells FACS analysis of MCF7 cells using GTX04863 NDP52 antibody [9E2F2]. Blue line : Primary antibody Green line : Isotype control Red line : Unlabelled sample Dilution : 1μg/1x10? cells](https://www.genetex.com/upload/website/prouct_img/normal/GTX04863/GTX04863_20240531_FACS_24053100_340.webp)
![WB analysis of various samples using GTX04863 NDP52 antibody [9E2F2]. Lane 1: Human placenta tissue lysate Lane 2 : HeLa whole cell lysate Lane 3 : Jurkat whole cell lysate Lane 4 : A549 whole cell lysate Lane 5 : Rat testis tissue lysate Lane 6 : Mouse testis tissue lysate Loading : 30μg Dilution : 0.5 μg/mL WB analysis of various samples using GTX04863 NDP52 antibody [9E2F2]. Lane 1: Human placenta tissue lysate Lane 2 : HeLa whole cell lysate Lane 3 : Jurkat whole cell lysate Lane 4 : A549 whole cell lysate Lane 5 : Rat testis tissue lysate Lane 6 : Mouse testis tissue lysate Loading : 30μg Dilution : 0.5 μg/mL](https://www.genetex.com/upload/website/prouct_img/normal/GTX04863/GTX04863_20240531_WB_24053100_867.webp)




![NDP52 antibody [HL3126] detects NDP52 protein by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. NDP52 stained by NDP52 antibody [HL3126] (GTX640612) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX640612/GTX640612_T-45467_20240806_IHC-P_24082802_845.webp)



![NDP52 antibody [GT1813] detects NDP52 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 protein stained by NDP52 antibody [GT1813] (GTX630397) diluted at 1:1000. Red: Phalloidin, a F-actin marker.](https://www.genetex.com/upload/website/prouct_img/normal/GTX630397/GTX630397_41589_20141230_IFA_w_23061202_490.webp)