
NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX630396) diluted by 1:500. The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
NDP52 antibody [GT422]
GTX630396
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetCALCOCO2
Overview
- SupplierGeneTex
- Product NameNDP52 antibody [GT422]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT422
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID10241
- Target nameCALCOCO2
- Target descriptioncalcium binding and coiled-coil domain 2
- Target synonymsNDP52, calcium-binding and coiled-coil domain-containing protein 2, antigen nuclear dot 52 kDa protein, nuclear domain 10 protein 52, nuclear domain 10 protein NDP52, nuclear dot protein 52
- HostMouse
- IsotypeIgG1
- Protein IDQ13137
- Protein NameCalcium-binding and coiled-coil domain-containing protein 2
- Scientific DescriptionThe protein encoded by this gene is a subunit of nuclear domain 10 (ND10) bodies. ND10 bodies are nuclear domains appearing immunohistochemically as ten dots per nucleus. They are believed to be associated with the nuclear matrix on the basis of their resistance to nuclease digestion and salt extraction. ND10 proteins are removed from the nucleus by herpes simplex virus-1 infection and may have a role in viral life cycles. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![NDP52 antibody [GT422] detects NDP52 protein by western blot analysis. A. 30 μg Huh7 whole cell lysate/extract B. 30 μg Hep3B whole cell lysate/extract C. 30 μg HepG2 whole cell lysate/extract 10 % SDS-PAGE NDP52 antibody [GT422] (GTX630396) dilution: 1:1000 NDP52 antibody [GT422] detects NDP52 protein by western blot analysis. A. 30 μg Huh7 whole cell lysate/extract B. 30 μg Hep3B whole cell lysate/extract C. 30 μg HepG2 whole cell lysate/extract 10 % SDS-PAGE NDP52 antibody [GT422] (GTX630396) dilution: 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX630396/GTX630396_41540_WB_2_w_23061202_768.webp)
![Immunoprecipitation of NDP52 protein from Jurkat whole cell extracts using 5 μg of NDP52 antibody [GT422] (GTX630396). Western blot analysis was performed using NDP52 antibody [GT422] (GTX630396). EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent. Immunoprecipitation of NDP52 protein from Jurkat whole cell extracts using 5 μg of NDP52 antibody [GT422] (GTX630396). Western blot analysis was performed using NDP52 antibody [GT422] (GTX630396). EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX630396/GTX630396_41540_20150317_IP_w_23061202_690.webp)
![NDP52 antibody [GT422] detects NDP52 protein at autophagosome? by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 stained by NDP52 antibody [GT422] (GTX630396) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10 μm. NDP52 antibody [GT422] detects NDP52 protein at autophagosome? by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 stained by NDP52 antibody [GT422] (GTX630396) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX630396/GTX630396_41540_20200205_ICC_IF_treatment_Chloroquine_w_23061202_426.webp)
![NDP52 antibody [GT422] detects NDP52 protein at autophagosome by immunofluorescent analysis. Samples: Hep G2 cells mock (left) and treated with 3 μM Thapsigargin for 16 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with 100% MeOH for 30 sec. Green: NDP52 protein stained by NDP52 antibody [GT422] (GTX630396) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm. NDP52 antibody [GT422] detects NDP52 protein at autophagosome by immunofluorescent analysis. Samples: Hep G2 cells mock (left) and treated with 3 μM Thapsigargin for 16 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with 100% MeOH for 30 sec. Green: NDP52 protein stained by NDP52 antibody [GT422] (GTX630396) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX630396/GTX630396_41540_IFA_w_23061202_409.webp)
![Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody [GT422] (GTX630396) diluted at 1:1000. Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody [GT422] (GTX630396) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX630396/GTX630396_41540_20160721_WB_shRNA_watermark_w_23061202_711.webp)
![NDP52 antibody [GT422] detects NDP52 protein by western blot analysis. A. 30 μg Jurkat whole cell lysate/extract B. 30 μg Raji whole cell lysate/extract C. 30 μg NCI-H929 whole cell lysate/extract 10 % SDS-PAGE NDP52 antibody [GT422] (GTX630396) dilution: 1:1000 NDP52 antibody [GT422] detects NDP52 protein by western blot analysis. A. 30 μg Jurkat whole cell lysate/extract B. 30 μg Raji whole cell lysate/extract C. 30 μg NCI-H929 whole cell lysate/extract 10 % SDS-PAGE NDP52 antibody [GT422] (GTX630396) dilution: 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX630396/GTX630396_41540_WB_w_23061202_735.webp)

![NDP52 antibody [GT422] detects NDP52 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 protein stained by NDP52 antibody [GT422] (GTX630396) diluted at 1:1000. Red: Phalloidin, a F-actin marker. NDP52 antibody [GT422] detects NDP52 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 protein stained by NDP52 antibody [GT422] (GTX630396) diluted at 1:1000. Red: Phalloidin, a F-actin marker.](https://www.genetex.com/upload/website/prouct_img/normal/GTX630396/GTX630396_41540_20141230_IFA_w_23061202_182.webp)




![NDP52 antibody [HL3126] detects NDP52 protein by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. NDP52 stained by NDP52 antibody [HL3126] (GTX640612) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX640612/GTX640612_T-45467_20240806_IHC-P_24082802_845.webp)
![ICC/IF analysis of PFA-fixed U2OS cells using GTX04863 NDP52 antibody [9E2F2]. Orange : Primary antibody Blue : DAPI Antigen retrieval : IHC enzyme antigen retrieval reagent for 15 mins Dilution : 5 μg/mL](https://www.genetex.com/upload/website/prouct_img/normal/GTX04863/GTX04863_20240531_ICCIF_24053100_480.webp)


![NDP52 antibody [GT1813] detects NDP52 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 protein stained by NDP52 antibody [GT1813] (GTX630397) diluted at 1:1000. Red: Phalloidin, a F-actin marker.](https://www.genetex.com/upload/website/prouct_img/normal/GTX630397/GTX630397_41589_20141230_IFA_w_23061202_490.webp)