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ProductsBack/NDP52 antibody [GT1813]
NDP52 antibody [GT1813] detects NDP52 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 protein stained by NDP52 antibody [GT1813] (GTX630397) diluted at 1:1000. Red: Phalloidin, a F-actin marker.
NDP52 antibody [GT1813] detects NDP52 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 protein stained by NDP52 antibody [GT1813] (GTX630397) diluted at 1:1000. Red: Phalloidin, a F-actin marker.
NDP52 antibody [GT1813] detects NDP52 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 protein stained by NDP52 antibody [GT1813] (GTX630397) diluted at 1:1000. Red: Phalloidin, a F-actin marker.

NDP52 antibody [GT1813]

GTX630397
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetCALCOCO2
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Overview

  • Supplier
    GeneTex
  • Product Name
    NDP52 antibody [GT1813]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT1813
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10241
  • Target name
    CALCOCO2
  • Target description
    calcium binding and coiled-coil domain 2
  • Target synonyms
    NDP52, calcium-binding and coiled-coil domain-containing protein 2, antigen nuclear dot 52 kDa protein, nuclear domain 10 protein 52, nuclear domain 10 protein NDP52, nuclear dot protein 52
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ13137
  • Protein Name
    Calcium-binding and coiled-coil domain-containing protein 2
  • Scientific Description
    The protein encoded by this gene is a subunit of nuclear domain 10 (ND10) bodies. ND10 bodies are nuclear domains appearing immunohistochemically as ten dots per nucleus. They are believed to be associated with the nuclear matrix on the basis of their resistance to nuclease digestion and salt extraction. ND10 proteins are removed from the nucleus by herpes simplex virus-1 infection and may have a role in viral life cycles. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NDP52/CALCOCO2 using anti-NDP52/CALCOCO2 antibody (A05876-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human U20S wholel cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDP52/CALCOCO2 antigen affinity purified polyclonal antibody (Catalog # A05876-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDP52/CALCOCO2 at approximately 52KD. The expected band size for NDP52/CALCOCO2 is at 52KD.
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