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ProductsBack/Nestin antibody [GT623]

Nestin antibody [GT623]

GTX630201
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetNES
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Overview

  • Supplier
    GeneTex
  • Product Name
    Nestin antibody [GT623]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT623
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10763
  • Target name
    NES
  • Target description
    nestin
  • Target synonyms
    Nbla00170; nestin
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP48681
  • Protein Name
    Nestin
  • Scientific Description
    Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Endogenous Neural Stem Cell Activation after Low-Intensity Focused Ultrasound-Induced Blood-Brain Barrier Modulation.
    Read more
  • Wnt/PCP pathway regulates the migration and neural differentiation of mesenchymal stem cells in vitro. Yao P et al., 2022, Folia Histochem Cytobiol
    Read more
  • Irradiated microvascular endothelial cells may induce bystander effects in neural stem cells leading to neurogenesis inhibition. Ma L et al., 2022 Mar 17, J Radiat Res
    Read more
  • Ten-eleven translocation 1 mediated-DNA hydroxymethylation is required for myelination and remyelination in the mouse brain. Zhang M et al., 2021 Aug 24, Nat Commun
    Read more
  • Long-Term Liraglutide Administration Induces Pancreas Neogenesis in Adult T2DM Mice. Deng H et al., 2020 Jan-Dec, Cell Transplant
    Read more
  • Lead exposure reduces survival, neuronal determination, and differentiation of P19 stem cells. Mansel C et al., 2019 Mar - Apr, Neurotoxicol Teratol
    Read more
  • Biomimetic 3D-printed scaffolds for spinal cord injury repair. Koffler J et al., 2019 Feb, Nat Med
    Read more
  • Biochemical re-programming of human dermal stem cells to neurons by increasing mitochondrial membrane potential. Liu H et al., 2019 Jun, Cell Death Differ
    Read more
  • Newborn dopaminergic neurons are associated with the migration and differentiation of SVZ-derived neural progenitors in a 6-hydroxydopamin-injected mouse model. Xie MQ et al., 2017 Jun 3, Neuroscience
    Read more
  • Improved Proliferative Capacity of NP-Like Cells Derived from Human Mesenchymal Stromal Cells and Neuronal Transdifferentiation by Small Molecules. Aguilera-Castrejon A et al., 2017 Feb, Neurochem Res
    Read more

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Figure 1. Western blot analysis of Nestin using anti-Nestin antibody (PB9874). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: HEPG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nestin antigen affinity purified polyclonal antibody (Catalog # PB9874) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nestin at approximately 230 kDa. The expected band size for Nestin is at 177 kDa.
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