
IHC (Free Floating) analysis of PFA-fixed rat hippocampus tissue using GTX00837 NeuN antibody. The NeuN antibody stains the nuclei and distal perikarya of most neurons, while the IBA1 antibody specifically labels microglial cells. Red : Primary antibody Green : IBA1 Dilution : 1:5,000
NeuN antibody
GTX00837
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRBFOX3
Overview
- SupplierGeneTex
- Product NameNeuN antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1,000. ICC/IF: 1:5,000-10,000. IHC: 1:5,000-10,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID146713
- Target nameRBFOX3
- Target descriptionRNA binding fox-1 homolog 3
- Target synonymsFOX-3, FOX3, HRNBP3, NEUN, RNA binding protein fox-1 homolog 3, RNA binding protein, fox-1 homolog 3, fox-1 homolog C, hexaribonucleotide binding protein 3, neuN antigen, neuronal nuclei antigen
- HostChicken
- IsotypeIgY
- Protein IDA6NFN3
- Protein NameRNA binding protein fox-1 homolog 3
- Scientific DescriptionThis gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2017]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
References
- Stress-mediated dysregulation of the Rap1 small GTPase impairs hippocampal structure and function.Read this paper
- An oligomeric semiconducting nanozyme with ultrafast electron transfers alleviates acute brain injury. Mu X et al., 2021 Nov 12, Sci AdvRead this paper
- Catalytically potent and selective clusterzymes for modulation of neuroinflammation through single-atom substitutions. Liu H et al., 2021 Jan 7, Nat CommunRead this paper







![NeuN antibody detects NeuN protein at nucleus by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NeuN stained by NeuN antibody (GTX132974) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX132974/GTX132974_44986_20230519_ICC_IF_R_23053001_624.webp)


![NeuN antibody [HL2194] detects NeuN protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. NeuN stained by NeuN antibody [HL2194] (GTX638198) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638198/GTX638198_T-44942_20230424_IHC-P_multiple_R_23050918_845.webp)
