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ProductsBack/Occludin antibody [MSVA-415M] HistoMAX(tm)
IHC-P analysis of human ovarian clear cell carcinoma (OCCC) tissue using GTX04461 Occludin antibody [MSVA-415M] HistoMAX?. Clear cell ovarian carcinoma with moderate occludin staining of tumor cells.
IHC-P analysis of human ovarian clear cell carcinoma (OCCC) tissue using GTX04461 Occludin antibody [MSVA-415M] HistoMAX?. Clear cell ovarian carcinoma with moderate occludin staining of tumor cells.
IHC-P analysis of human ovarian clear cell carcinoma (OCCC) tissue using GTX04461 Occludin antibody [MSVA-415M] HistoMAX?. Clear cell ovarian carcinoma with moderate occludin staining of tumor cells.

Occludin antibody [MSVA-415M] HistoMAX(tm)

GTX04461
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetOCLN
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Overview

  • Supplier
    GeneTex
  • Product Name
    Occludin antibody [MSVA-415M] HistoMAX(tm)
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:100-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MSVA-415M
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID100506658
  • Target name
    OCLN
  • Target description
    occludin
  • Target synonyms
    BLCPMG, PPP1R115, PTORCH1, occludin, phosphatase 1, regulatory subunit 115
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ16625
  • Protein Name
    Occludin
  • Scientific Description
    This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Occludin/OCLN using anti-Occludin/OCLN antibody (A01246-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Occludin/OCLN antigen affinity purified polyclonal antibody (Catalog # A01246-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Occludin/OCLN at approximately 65KD. The expected band size for Occludin/OCLN is at 59KD.
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