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ProductsBack/Oct4 antibody

Oct4 antibody

GTX100468
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetPOU5F1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Oct4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. FACS: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.62 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5460
  • Target name
    POU5F1
  • Target description
    POU class 5 homeobox 1
  • Target synonyms
    OCT3, OCT4, OCT4Borf1, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4, POU domain, class 5, transcription factor 1, POU domain transcription factor OCT4, POU-type homeodomain-containing DNA-binding protein, octamer-binding protein 3, octamer-binding protein 4, octamer-binding transcription factor 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01860
  • Protein Name
    POU domain, class 5, transcription factor 1
  • Scientific Description
    This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewings sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Gupta A, Storey KB. Activation of the Hippo Pathway in Rana sylvatica: Yapping Stops in Response to Anoxia. Life (Basel). 2021,11(12). doi: 10.3390/life11121422
    Read this paper
  • Yao X, Zhang M, Wang X, et al. Tild-CRISPR Allows for Efficient and Precise Gene Knockin in Mouse and Human Cells. Dev Cell. 2018,45(4):526-536.e5. doi: 10.1016/j.devcel.2018.04.021
    Read this paper
  • Sundararaman B, Zhan L, Blue SM, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016,61(6):903-13. doi: 10.1016/j.molcel.2016.02.012
    Read this paper

Datasheet

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Figure 1. Western blot analysis of POU5F1 using anti-POU5F1 antibody (A00174-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POU5F1 antigen affinity purified polyclonal antibody (Catalog # A00174-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POU5F1 at approximately 45 kDa. The expected band size for POU5F1 is at 39 kDa.
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